FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_WRKY40_col_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_WRKY40_col_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCTTTTTTAGCTATACATTCATTTTCACAACAAATTTTTCCAAGAC11.282051282051282No Hit
AAGAAATACTTGGAATTTGAAAATTCTTTTTGGCATGCATCATAAGGATA11.282051282051282No Hit
GAGGAGATTGTATAAATAAGCTTATAATTTTGTACGTTTCTCGAGATAGT11.282051282051282No Hit
GATCCAGTACCAAGAAGAAGAAGACACCCATGACAAACAGCAGAGGACCA11.282051282051282No Hit
TAATAATAATAATTTGTCAGTTTTATGTCTTAATCTGTTTTCACTACGTA11.282051282051282No Hit
ATCTGGTTATGCTCATGCCCGGTCCTGTTTACATGCTATGTAATATATGA11.282051282051282No Hit
GCTACACCATGAAAGCTGTGAGAAGCAAGAAGAAGGTTGGTTAGTGTTTT11.282051282051282No Hit
AAAGAAGCAAGACAATGGATCTTTTCGATTGACTTTTAGTCGACTCCAAA11.282051282051282No Hit
TTCTAGTAAAAATGTTGATGTAAAGATGAAAACTTTCCCCGAAATCTCCT11.282051282051282No Hit
TGAGGTTGCTTCGGTCATTTTGTTGTAAGGTCTATATAAAGACCAAATTT11.282051282051282No Hit
CAATGCTTGAAAAAGTAATTTTGGGTCCGTATTATAGCACAAATCACGAA11.282051282051282No Hit
GGATATAGTCATGATTCGATGGGTTAGGTCCACTTACTTTTTCTTTTGGA11.282051282051282No Hit
ATACATTCACCTTAAAAAGTAAACATGTTCATGTGATGTTCAAGGGAAAT11.282051282051282No Hit
CAAAATCAAATTAATAACTTAGTCACAAGGCTAAAAATTGTTTTTTCAAT11.282051282051282No Hit
CAACTTTAACCTCCAGCGATCGCACTTTCTCCTCCGACGCGAATGTCCAT11.282051282051282No Hit
AATGTTGCATCATCTCTAAACATTCTTCTGTTTGTCTAGTGAAACAAAAC11.282051282051282No Hit
ATTATCGTACTGGCCACTGTTAAAGCAGGAAACTGTGAACGAAGGAAGGA11.282051282051282No Hit
CTAGACCATGAAACTCCGATGTTGTATCACGTCAAATGACCTAGCTAGAG11.282051282051282No Hit
TTAGTCTGCTAGAAAAAGCATGTTGGAGTGGATTTATTGAATAAAGTCAC11.282051282051282No Hit
ATGCCTCTAATGTGTGGTAAACCTTCTGGATTTTCCGCGGGAAAAACATA11.282051282051282No Hit
TGTACGTGCTCTGATTCTTGAAGTCAACTAGAACCAATCTGGTCGTAGAC11.282051282051282No Hit
AATTATGGAGGGTCTTCAGACAGTCCCCAAATGATGCAGAAGCGAGGGTC11.282051282051282No Hit
TATAACTAAGGAATTTATGGCACCCTAACCCTGGAAACTCTGGCTTATAT11.282051282051282No Hit
GTTTTGGGATTTATGTGTAGGGACTGTGGAAAACAAGTTTATTTGGGTAA11.282051282051282No Hit
TCATGTCAGCAGTCTACCAGACGGTGTTCTTTGCAATATATTTTCTGTCC11.282051282051282No Hit
CATCAGATTGCATCATTGACTCCATCAAATTCTCTGACATCCATCTCTGT11.282051282051282No Hit
TGGATTTAACGTTTTCATTTGATGAAGTCGTAAAATCTCAGAGTTTTGAC11.282051282051282No Hit
TGAAGCCATGAGTTCAATGTTGCAATGCGTCTTGATTATTGACTTTACCC11.282051282051282No Hit
AGTCGTCAAGATCGCAACCTGTGAATCGGAGGAGAGAAGACATTATAGCT11.282051282051282No Hit
AAAAGAAAATTGATAAAGTCAAGTTGACCTAACTATTTTATGATTATGTT11.282051282051282No Hit
GAAGTTGAGTCAACGCATTCAATGAAGAATCATTGATGGTCGTGTTTAGT11.282051282051282No Hit
CTTGCGAAAGGACTTTATCATTTGTGGAATGAATACTTAGGGGAAAATAT11.282051282051282No Hit
AAGGAGTCATTCTTCCATCAAGACAGGCCTTGACTTCCGAGTTCACGATT11.282051282051282No Hit
AGACATGTTGTATTTGGTGCTTAATGACTAGGTAATGAAGGCTATATGTG11.282051282051282No Hit
CAATTATTTGAGTTAGTCGTTGTCTATTCTTACATAACGGTCCAAGTTTG11.282051282051282No Hit
CTACGGCTTCTTGACGCTTCTACTTCAAGACGATGTCGAAGGTCTTCAAA11.282051282051282No Hit
CTAGTTAATTTATAATGCTAACAAACAGAATCAAAAGAATCAAAATCCCT11.282051282051282No Hit
CTACATATGACATCAAGTAAAATTAGAATCCAAAACCCTAACCAACCTTT11.282051282051282No Hit
AGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGAATTAGATC11.282051282051282No Hit
TATAGACTCTGAATTTCTTACAAGGTCACACCTAAGATTATTGAAAAATA11.282051282051282No Hit
GTTTGGAGGTATCTCATGTCTTCTAAAACTGAAGTTTCTCAGCTCATTAA11.282051282051282No Hit
AGAAATTCAAACAAAGCAGATGACAAAGCTGAGAGCTGACTAGAAAAATT11.282051282051282No Hit
TTTTTTTTTTTTGTCGGCCTAAATTTTAATAAATTTCGAGTTCATTTATG11.282051282051282No Hit
CTCACCATGTCACGGAGACCCATAAAATACATATGTTTGGCTCATGAATG11.282051282051282No Hit
ACTGGCACAAGTATATCGTTTATGGAACCTGGAACCCATTCGTGTAGCCT11.282051282051282No Hit
CTGTTTAGCTCTCTGGAGTTCATAGATTGGATATTGAATTATCCGAAGAT11.282051282051282No Hit
ATTCCTTTACTTCAGTCTGTTGAGACTTGGCTATGAAGTTGTTTTTCAAT11.282051282051282No Hit
ACCCTGATCAATAAACAGATAAAACCCTTCAATTTGTATCTGATTAAATC11.282051282051282No Hit
TATGACGTCATTATAATGTATATTTGTTGAGGTTTTGTTCTTATAAATGT11.282051282051282No Hit
AATAATTACAATTATATCACTCTATTTTTCTAATATCATATGATTGTTGA11.282051282051282No Hit
ACTAAGAAGAGAAAGATTTTCTCTTCCTGGTTTTTGATATATTTTATTAT11.282051282051282No Hit
AGAGATTTTTCTTACAAAAGTTTTATAGTTTGAACTAGTCATGTCTTAGA11.282051282051282No Hit
CAGTAGTAGTCCAGAGAGAGAAGGAGAATCTTCTTCAGCCTCTGGAAGAG11.282051282051282No Hit
CACATTTTTACATATATTATTTTCATTATGCACCTAAAGGATGAGCCGTA11.282051282051282No Hit
TTGACGTTGAATACTCAATGTGGTTGTCCATAAGAGAAGGAACAACCACG11.282051282051282No Hit
AGTGTTTGAGTTTTCACCAACTACCTTGTGTTTCATCCCTCATTTGATGC11.282051282051282No Hit
TGATATACATGCCATCAAGTCATATTCGCCTCCTAAACACTAACCAACCT11.282051282051282No Hit
TGAAAGTCATAAAAGTGAGTCACAAGAGGTATAATAGATCGGAAGCGCGG11.282051282051282No Hit
ATAACTTCATTGCTAACTTGAATTTGCTTGACCAGATCGGCAGTTTTCAG11.282051282051282No Hit
TTCAAGATTCTCAATGCGCTAAGACGTTTCTATCACTAGATTTTCACAAA11.282051282051282No Hit
AAGCAACCTTCTTCTTGCTTCTCAAAGCTTTCATGGTGTAGCCAAAGACA11.282051282051282No Hit
GCAGTGTTCTTACTTGTCCTGCACTTCGTGGACAGTGGTCAGGACTCACT11.282051282051282No Hit
GTCCGAGACTTCATCGACCGTGTCCGAGGAGTGGTCGACCAGGAGTTGAA11.282051282051282No Hit
TTTAGAAGTGAGTCAAGTGACATGTATGGTTCATATGAAGACTCACGAAC11.282051282051282No Hit
CTGGCATTCTCAGTCTTTTGACTTTCTCATGGATGAGTCCATTAATAGAA11.282051282051282No Hit
ACGTAGCTTTAATGGACGGTGGATCTTCGCTCGTTGATCTTCTAAAGTTT11.282051282051282No Hit
CATTCATCGTCCTGGTCGAGCGTTCCGCCGACCTGGTCGACGAATTCTCG11.282051282051282No Hit
CGCCAAAGTCCGTATGAGTCTTTGGATTTGTATCTTCTAACAAGGAAACA11.282051282051282No Hit
GTTTTCACCTACATAATTAGAATTTTTCCGTCCAAATTGTTTTTGGTCAA11.282051282051282No Hit
ATTATACTAAACAAAAATCGTGGTTAGAATTCATTTTTCATGTACCTGTG11.282051282051282No Hit
ATCGAACCATATAAATGTTGACTAATCTGGCTAACATTGAACTTGATAAA11.282051282051282No Hit
ATGGGAACTTGATGAGTTGACCCGCCTACACGTCTTGCTTTTACTGCTAT11.282051282051282No Hit
TCGACGCATTCTCGGTCACGGTCGATCAATTCATCTCCTGGTCGACCAAA11.282051282051282No Hit
CTGCTAGTTTCCCTTCTTTGTTTCCCAAGAATTCAAAATCAACTTCATCA11.282051282051282No Hit
AATGCAGCCAAGGACACCTTCAAGGTCTCAAAAAAAAGTCAAGAAAATTT11.282051282051282No Hit
ATTCTCGGTCCTGGTCGACGATTACAACCCCTGGTCGACAAATCCTCGGA11.282051282051282No Hit
CCTTTCAGAAGGTAAGTCACACAGGTAACTGAAAGATGGGTCGTGGTAGA11.282051282051282No Hit
AATAAAGCTCGTTGTGTTTATTTGTTTTCTTTTACTTAGGTACTTGAATA11.282051282051282No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGACC50.070.014
GTTTGGC50.070.035
CATGAAT50.070.043
GTCACGG50.070.09
CGGAGAC50.070.013
AAATTAA50.070.055
ACCATGT50.070.04
TATGTTT50.070.032
TAAAATA50.070.023
GTCAAAG50.070.064
CCATGTC50.070.05
AAATACA50.070.025
TGTTTGG50.070.034
ATGAATG50.070.044
GGATCAA50.070.050
AATTAAT50.070.056
TGTCACG50.070.08
GGTCAAG50.070.070
CCCATAA50.070.019
GAATGGA50.070.046
Produced by FastQC (version 0.11.5)