FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_WRKY40_c24_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_WRKY40_c24_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5778945
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGG9600.016.4061270
GTAGGGG6553.1468517E-1010.15259270
GCGTCGG8101.6552804E-109.07400370
CGGAAGC18350.08.39230470
TCGTGTT38200.08.06276470
AGTGTAG42250.07.04136470
GAGTGTA47550.06.33012969
AGAGTGT56850.06.21806969
AGCGCGT9255.7387973E-56.05400660
GTGTAGA41450.05.995127770
CGGAGGG8852.0443923E-45.93220865
GGGAAAG91400.05.85886667
TAGGGAA107350.05.73819470
GCCCAGG10455.4372867E-55.69378422
TCGGTGG11708.57003E-65.68376567
TGTAGGG126900.05.68159470
GTGTAGG139950.05.676982470
GGAGGGC8659.785561E-45.66474466
CGTGTAT34150.05.63685270
GTAGGGA108800.05.597382569
Produced by FastQC (version 0.11.5)