FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_WRKY40_c24_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_WRKY40_c24_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5778945
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGG9700.016.2369870
GTAGGGG6600.011.66657270
GCGTCGG8151.8189894E-129.8772270
CGGAAGC18500.08.32425670
TCGTGTT38200.08.06276170
AGTGTAG41900.06.84958670
CGGAGGG8904.581363E-66.68539665
GGAGGGC8652.154496E-56.473991466
AGAGTGT56150.06.295585669
GAGTGTA47000.06.255268670
AGCGCGT9255.738817E-56.054004760
TAGCGTC7000.00339551226.000003362
GGGAAAG89700.05.969902567
TCGGTGG11355.1309944E-65.859033667
GTGTAGA41150.05.783670470
TGTAGGG125200.05.75873970
TAGGGAA105800.05.689934770
GTGTAGG137950.05.68317270
GAGGGCT8800.00121708165.568184467
CGTGTAT34650.05.5555170
Produced by FastQC (version 0.11.5)