FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_WRKY40_c24_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_WRKY40_c24_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAAAGGTCAAAATTTGGAGTCGACAAAAAGTCAATGGAAAAGTTCCA12.3255813953488373No Hit
TGACCGACCGTTAAATCAGTTTTCTAGCCTAATATTTATTAGTTTATTCT12.3255813953488373No Hit
GGAGTCAATTACGACACTGTGATTCCGAAGCAGGTCCTGATACCCGCTCA12.3255813953488373No Hit
CAAGATAAGCTTGATAATTTGAATTGATTATAAATAATGGTAAATATGAA12.3255813953488373No Hit
AGTCCACTACCATGCCGTCAGGTATACAGAGTCTATCAACAGGATGGGCC12.3255813953488373No Hit
TTTGACGTAGTTGCCCAAATCAAAGCTGAACTGGAGAAAGCATGCCCTGG12.3255813953488373No Hit
AGTTGCTGAGCTCTGCGTGCTGAGATTGAACTGTCTTGACCACTTAAACC12.3255813953488373No Hit
ATCAACATGATATAGAACTTCGACCCCAACTCCTCCTTCTTACTTGAACT12.3255813953488373No Hit
ACTGTTATGTAGACTTTTGTTTACCGACGGTTCGAATCCCTCTCTTTCAG12.3255813953488373No Hit
ACAATAGACAAATAAATCAGTAACTCAGCTGCATCTTATCCTTATAGCCT12.3255813953488373No Hit
TGTTTTAGTCAACATTTTTTTTTTGTCAACCTTTTAAATGGTAGATAAAA12.3255813953488373No Hit
TTCCTCTACCCCAGTCAACAAGACAGAGACCCTGAGGTAGAAATGAAAAG12.3255813953488373No Hit
AAATCGATGCCTTTAAATGTAGTCAATGATCGTGATGGCTCTTCTCGCAT12.3255813953488373No Hit
GTCCGAGGAGTGGTCGAGGGTTTGTCGACCAGGACGCGGTCTCGTCGAAC12.3255813953488373No Hit
ACCTTGAATGGACTTGTGACCAAAAACAGAGGATGTGTTTTGTTTATTCT12.3255813953488373No Hit
CAGTTGAATAGAAAGTCACTATCTTTTAAAAAGTAAGCAGTTGATTGTTT12.3255813953488373No Hit
TAAAACTTTATGACTCTATAACTTCTATACCGTGAGGCCAACCATGGCAT12.3255813953488373No Hit
AAACTTTTCTTTCCACAGAGCCAGGCTGATTCATAAGAGGGGCACCACCA12.3255813953488373No Hit
CTTATGAACGTTTGACCTCACTGACAAGCTAGTTTCTCTATGTAATGTTG12.3255813953488373No Hit
TGAGTTACGGTATGTTGCGGCTGACTTACGTCAAAAATATATGAGTCATA12.3255813953488373No Hit
GAATATACAAAATTTATATATTATTTTTATTTGTGGGCACAAAAAACTTA12.3255813953488373No Hit
ATCTAGCCATATTTGATTCCGAAAAACTAACCAAGCTACTTATTGCCTCA12.3255813953488373No Hit
AATGGCGACTTACCATTCTGTGAAGAATTTCTAGATACATAAAATCAAGA12.3255813953488373No Hit
TTCCAAATCCAATGTCGTGACCCTGGCTAGCTGTTTGTCACTCCGTTTGA12.3255813953488373No Hit
TACTAGGATGGGTGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTCTTTT12.3255813953488373No Hit
ATAAAAGAAGCCTTCACTTGACTTTAGAGCTTCCCTGTAGTGCTGATAGT12.3255813953488373No Hit
TCTGTTTTACTGAATTTTTATGGAGTATCTCTAATTCGAGTAGTCATACT12.3255813953488373No Hit
AAAAAAGGTGAAAACTTCAATTCAAAGTCTAAGTCTTTCGATGGTTTATC12.3255813953488373No Hit
ATTTCCACTAATGTTGGAATAAATGAATTATGACTTGACCAACTTATCCA12.3255813953488373No Hit
AGCAGATAATAAAATATAAAGATTGGTCGATTTATCATGGTATAGTACAT12.3255813953488373No Hit
AACATATGTTTATCAATATCAAGAAAATTTACACAATGTGCCAGTTGTAT12.3255813953488373No Hit
CTTTATGGATGATTTGAATCTTATGCAGCGTCTCTCGAGACCCTTTGCAA12.3255813953488373No Hit
AAGAGCTTCCATGGTTGGAATTGAAGTCTAGCCTGACAGTGTTGACAAGT12.3255813953488373No Hit
GAACCATTGATTAATTCATTCCTTAGAGTATGGGTTCATGCTTGCATATA12.3255813953488373No Hit
CTGTTGTCGTGCCGCTTTGGTTACTAAGGTTACCGGGGTCTAGCTTTAGG12.3255813953488373No Hit
GTGTATATATATAAAGGAGTCAAGTTAGTCAAGCTTCATAAGGATACATA12.3255813953488373No Hit
AGTTCCCTTTTTGCTTCAACCCGTCAACTAAAAAAAGGGTATAAAAGGAA12.3255813953488373No Hit
GCTTCTACTATAGAATACATCTGTGAGTCGCACACACTCGTTGACCGGAA12.3255813953488373No Hit
CACTAACCAAGCTTCTTCTTGCTTCTCAAAGCATTGATGGTTTAGCTGAA12.3255813953488373No Hit
CAACTACCTGCCTCTTCATAGTACTGTTTTATTCTAGTGAGAAAACATTT12.3255813953488373No Hit
TATAGTCAATCGCTCTCGATCACCGAAAGATTCTAGGGACTAGATACAAG12.3255813953488373No Hit
TGCTTTCTACCACTAGCTAAGAAAAACGACTAGAGCTTCATTTTTAGCAT12.3255813953488373No Hit
CAACACATATTCCGATTCAAGCTACATGATGCTTGAAAGGCCATGGAAAA12.3255813953488373No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)