FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_WOX11_col_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_WOX11_col_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3267284
Sequences flagged as poor quality0
Sequence length76
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG1358.09996E-925.92612570
CGCGGAG850.003792069920.58839261
CGCGTCG2408.176241E-411.66675770
AGAGCGG3004.4425967E-410.5000870
TGTAGGG33750.06.2222769
GTAGGGA28550.06.12964470
AGTGTAG15152.226625E-85.775621467
GTGTAGG36850.05.698822568
CGTGTAG41600.05.468791568
TCGTGTT14207.3266474E-65.17609670
AGAGTGT18951.875378E-85.171543670
GTCGTGG9700.00412947365.05158570
TCGTGTA56550.05.013301466
CGTCGTG68450.05.010995470