FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_WOX11_col_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_WOX11_col_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length76
%GC29

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGAATAGTCACCATAGACTACGACCCTAATCGAACTGCATACATTTGT15.0No Hit
TAGTAGTTTAAGGTTTTTGCACACAAATTAATAACATATATAACTCTCAT15.0No Hit
TATGTATTTTTCTTAATTACAAATTTAGAGATGAAAAAGACACAATATTT15.0No Hit
AAACATTTGACATTATATAATTAACCAATTGATTTTAGGATAGGACTCTA15.0No Hit
AAACTGTTCAAAGTTACTACTTCGTTTGACGAAAAAAGAAAAAAAAAGTT15.0No Hit
CAAAACCCAAATTCCCAAAAATCCAGCTAAAAGCAATAAAATTCTGCAAA15.0No Hit
AATGTAGTAATAGTTAAATTAAACTATCTGTTTTGTCTAGTGTATGAGGA15.0No Hit
TACTTCATCCATTCCATGAAGATTGACCTTTTAATTACGTTTTTATTTTG15.0No Hit
TTTAAATTATATTATTATTTTTATTAAGTTATTATGAATCAAATATTTAA15.0No Hit
CTACTTAATGTCTTTGAATACCCCCAAACTTGTTTCTATAGTCAGGATTA15.0No Hit
CTTTGGATATGATAATTTTTTACTAAATATGTTTTGATTGTTACAGACTG15.0No Hit
AATCAGGACCTGACATGAGAACAAGTACCCCCCACTGGAGTGTCAGAGTG15.0No Hit
AAAAGAAAGTACAGAATGGATCATCACTAGCAATCGCTCAAGGCTTCCCC15.0No Hit
AGCATATTTTGTCAAGTAAATATTTATCATATTTATTTTTTTAAGAAACC15.0No Hit
ATAATGCTAGTTTAAATATATATTCAAATATGTTTAATTAATATTGTGTT15.0No Hit
AGCTAATTGTAATTAAATAGTATTATAAACTTTTGAATTATCATATATAT15.0No Hit
TAAAAAACTAAGACATTTTTTATATAATTTCTGCGGCGCATCCAACTAAA15.0No Hit
CGATGTAAAGCATTTCGTTAGTTTAATAAACGTCCGATATGTTTCTTTGT15.0No Hit
CATTTACAAATTTATCGAGCAAGATTGGAGTTACGCCAAGCCCCTCCATC15.0No Hit
TTTGGATTGTGTAGATGAATGTGTTAGTTACCTAATTGCCTTCGAACATC15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)