FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_WOX11_c24_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_WOX11_c24_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length76
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATCAGGTTACATATATTAAGCTAATGTTCATGATCAGATTCTATGAT11.9230769230769231No Hit
ATCGGAGGCATAAAACAATCTTAATGTAAACGTCTTTAGACGATACAGCT11.9230769230769231No Hit
AACAGAGCTTTAGTCAACTTATCTCAACAAACCAAGAATCAACAATTAAA11.9230769230769231No Hit
AAACAATTATGCAAAAAAACACAGATAGAGAAGAGAATATTCCTCCAACG11.9230769230769231No Hit
TTGTTGATGATGTAAATAGGTTTAGTAATGCTACGAAGTAACTTATTAAA11.9230769230769231No Hit
TTAGGCACTTGAAGAGAATGTGAAAGAAAACTCCCTCAATCTTGACATGT11.9230769230769231No Hit
GTGATATCTACTTAAGAAAGGGTATGTGAATAATGAAATCTGCCCTTGCG11.9230769230769231No Hit
AACTCTTTCTTCCTCTGAAGTAAACGTGTTCTGATGTAGAAGAAGACAAA11.9230769230769231No Hit
ACCGAAGTTGAGATGATCAGTATGGCAATTCATAGGACCTTGCTTCTGCT11.9230769230769231No Hit
AGTAATTTTAATAGTACGTACGCTATATAGCACGAACTACTCACCCCTGA11.9230769230769231No Hit
GTAACTAATCCTAACATTGAAGTATTAAAAAAACCCATATAAGCAAAAAA11.9230769230769231No Hit
TTTAATACTTATTATCATATCACGAACAACAATTACAACATATAGTTTTA11.9230769230769231No Hit
GAACTAATATTTTTAATATGGAATTGGTTGGGCCTTTTGGAGGAGAAAGG11.9230769230769231No Hit
AAGAGATTGACTAGCAACTACATCTCTAATGTCAATGACACCAAATGGAT11.9230769230769231No Hit
TTTAAGTTTTTTTGATTTAGTAACTATTTAGTTTTAAGTTTTGCTTTACA11.9230769230769231No Hit
CTCCCTCCGCCACGAATCTCGAGGGGTCTTCTGAATGATTAGATTTCTGG11.9230769230769231No Hit
AAATGTTTGAATTGAGAGTTCGTTCATACGTCAAGATTTTTTGGATCTTT11.9230769230769231No Hit
AAAATGTATTCTCTAAGAGAAGGAAAACCTAAAGAAAATTAATTTGGAGT11.9230769230769231No Hit
GATATAATTCAATTACCGGTATGAGGCTTGGGTCTTCTTTTGCACAGCTA11.9230769230769231No Hit
GATCAGCGCCTAAGGTTCTTTAATGGCGATGACGAACGAAGCAAGCTTGA11.9230769230769231No Hit
CAATAAGAAGAGAGCAAATAATGGAGCGACTTTTTGTTCTACTGTTCTCG11.9230769230769231No Hit
CTAAAAAAAAAGAGAATAGATTCTCTATTTTTATATCAAATATCTAATTT11.9230769230769231No Hit
TCAACTCAACCGATACGAACTGCTGCACGGTTTGGTTTGTGCCATTATTA11.9230769230769231No Hit
TCTAATAGCGGACTATTTAAAATTTTAGTTTTTGTTGTTTGCTGTAGTTA11.9230769230769231No Hit
AGAAATTATATATTTAAATTGTAGATTTTTAAATGGTAAGTAATCTAACT11.9230769230769231No Hit
GTACAGCAAACTTTGCAGTTATGGGTCAGTACTCAACTGCAACAAAAAGA11.9230769230769231No Hit
GAAAAGAACAACAGTAAGCAAACTTCATCGTCATGTTCCTTTTCAAGATC11.9230769230769231No Hit
TTAATGATTACTGTCTTAAATATTCATTTTGAAATTATGATTACTTCACT11.9230769230769231No Hit
TTTAGTTTAAAATGAAAACTAAAATGTTAGAAACCAATTGTAAAAGAACA11.9230769230769231No Hit
TTTTCCTTTTCTTTTTAAATCAACTTTAACTATCTACTTGATTATTTTGA11.9230769230769231No Hit
AAAAGAATAATATATGAATTAATCGTGTTAGGGTTTAAGGCGTTTGCTGT11.9230769230769231No Hit
AAATATGGAAAAGCCGGCTATCGGAATCGAACCGATGACCATCGCATTAC11.9230769230769231No Hit
TTTTGACCCAAAACAAATATAAAAAAGTAACCATTTCCTTAGTAAAACGG11.9230769230769231No Hit
ATAATATAAACTCAAAAAATAACAGTCGTGTTTTTATTTAAATGTTATTT11.9230769230769231No Hit
CAATCAAAAAGACTAATTTAGTAAGTTTACTACCCTCTAGTGTTAGTTTC11.9230769230769231No Hit
GTGTTACGTTTTTCTGATATATGATAAATTTTACAAATAAGACTAGCCAT11.9230769230769231No Hit
GTCAAGAGAAAAATACATACAGGTTAAATCGATCTTCATAGGTGATAAGA11.9230769230769231No Hit
ATTTGTAACAAACTAATTTGATTGACAAAACCATATACTCAGTTGATGAT11.9230769230769231No Hit
CACACGCTTACAAACACTAAACAAATCAGTAAGGTCCTAGACCTGATATG11.9230769230769231No Hit
ACGCAAAGTGAGATCAAACGTATACCTAAGAAGGGACTTGGGGAGAATTG11.9230769230769231No Hit
GAAGATATTAAGTATCTGCCTACTTCGAATAAAGTGGAAGATGATATATA11.9230769230769231No Hit
ATTTTAGTTTTTATGTTTTTTAGGTATATAAATTAACTATTTTGTGATCT11.9230769230769231No Hit
GTGATCCTTAAAATTCTTTTAGTCACCAAGAGGAGTTCGAACTTTCTAGA11.9230769230769231No Hit
TTCTAACTAATGCTAAAAAGTCGCTAGATTACATGTTTAGGTTAATTTTG11.9230769230769231No Hit
GAATTAAATAATCAGTTGAATATTCGGGAATTAAAAACTCGATAAATTCA11.9230769230769231No Hit
TAGCTTCTGACCCCATGGGGAATTTTTTTAATTACTTGTATGATAATTAT11.9230769230769231No Hit
CATTTTAAATCATTTTTTGGTATAAACTTAAACTACAAATCTTATATAAT11.9230769230769231No Hit
GGAGTTACAACAACACTTAAAGATATTCAGGTAAAGCTATATCTTTTTTA11.9230769230769231No Hit
TTCTCAAAGCTTTCATGGTGTATCCAAAGTCCAGATGACTCTTTACCTAT11.9230769230769231No Hit
GCAATATTTTTTCTTAAATTTGATCGATATATTCTCTTTCCCTAATATAT11.9230769230769231No Hit
CACTATGATTTAATATTCACAAACTTAACTAATTTAGTGAATTATATAAA11.9230769230769231No Hit
ATCTAATATCAATAAAAATATATATGATAAATGTAAAATAAAATTTTATG11.9230769230769231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCAT50.070.010
TTCATGG50.070.012
GTCATTT50.070.053
AACAAGG50.070.060
AGCTTTC50.070.08
GTGTCAT50.070.051
CCTATGT50.070.046
CTTTACC50.070.041
TTACCTA50.070.043
ATCCAAA50.070.022
ATGACTC50.070.035
TGACTCT50.070.036
GTGTATC50.070.018
GATACAA50.070.066
CCAAAGT50.070.024
AGGATAC50.070.064
TATGTGT50.070.048
CTCTTTA50.070.039
CCAGATG50.070.031
TTTCATG50.070.011
Produced by FastQC (version 0.11.5)