FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_TGA5_col_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_TGA5_col_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160234
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG3202.2842869E-814.21876670
GCGTCGG5200.013.46155470
CGCCGTA2559.823749E-512.35295647
CGGAAGC15600.011.66667970
GTAGGGG5602.2191671E-1011.25001270
TCGGTGG9600.010.57292869
CTCGGTG10050.010.44777370
TCTCGGG4905.257334E-710.00001170
GGTCGCC3904.4914625E-59.87180570
AGAGCGG8151.8189894E-119.44786370
CGGTGGT8403.45608E-119.16667770
TCGTGTT33000.08.590919570
ATCTCGG15200.08.51974668
GTAGGGC4250.00114565948.23530465
GATCTCG16150.08.23530370
TCTCGGT13500.07.777786369
AGTGTAG54500.07.577990569
GAGTGTA55250.07.411772769
GTGTAGA49000.07.14286570
TGGTCGC5450.0016606327.064227669
Produced by FastQC (version 0.11.5)