FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_TGA5_col_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_TGA5_col_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160234
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGG3251.7262209E-915.0769470
GCGTCGG5300.013.8679470
CGCCGTA2559.823749E-512.35295647
GTAGGGG5702.5465852E-1111.66667970
CGGAAGC15650.011.62940770
TCGGTGG9750.011.12821769
GGTCGCC3954.453648E-610.63292470
CTCGGTG10150.010.00001170
TCTCGGG4956.041155E-79.89900170
AGAGCGG8201.8189894E-129.81708470
CGGTGGT8555.456968E-129.415215570
TCGTGTT32900.08.29788270
GATCTCG16150.08.23530370
ATCTCGG15400.08.18182868
GTAGGGC4300.00128615148.13954465
TCTCGGT13650.07.692316567
AGTGTAG54650.07.621234469
TGGTCGC5552.4306792E-47.56757669
GAGTGTA55600.07.428065369
GTGTAGA49200.07.184967570
Produced by FastQC (version 0.11.5)