FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_TGA5_col_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_TGA5_col_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAATGGTTGAAATTGAAAAAACACTTACTCATTTGAATACTTATTAT14.761904761904762No Hit
CTCTCATTGCCACAACATATTCATAAGTTGTAATGCCCTATTAGTGTTAA14.761904761904762No Hit
ATTATTATTGCTTGTCTCTTAAATTAGTTCTTAGGTTAAGAACATCTCAT14.761904761904762No Hit
TTCTTGTGCAAGTAGAAGATTGTGATAGTTGAGAGGATCTATTGGTACCT14.761904761904762No Hit
AGGCAAAACCATCACTTTGGACGTTGAAAGTTCTGATACTATTGACAATG14.761904761904762No Hit
ATACTATAAATAACAACACAAACTTTCGAAAATAGGAATAATACTTTGAT14.761904761904762No Hit
TGGAAACATAAACCTAAGTCAACTAATTAGTGAATAATTTCCGGAATTAG14.761904761904762No Hit
ATTTGTGTAGAGCACACAAATTCATTTTCTACAACTGACATATTCTTATC14.761904761904762No Hit
TTATGGTCGAATGGTTTTGTAAGAATATGGAGATGGAGAAAGCTATTGAG14.761904761904762No Hit
AGTTATAGTATGTTCGAACGTGGAATTCATCATCCTTATTAATTTTGTCT14.761904761904762No Hit
TTGTGCGACCTCAGCTTAAGAATACGTTGTTTGATTAGAAGAGGTGAATC14.761904761904762No Hit
AAAGTGGTCAGCAAAAGAAGCAAGACTTTCGTAACTATTATTTTATAAAC14.761904761904762No Hit
AAGATCACAAGATTTTTAACCAAAAAAAAACAAAACACAGTTCTCATTAC14.761904761904762No Hit
AGAAGATGGTTGGTTAGTGTTTTGGAGTCTACTATGACATGATCTCCTGT14.761904761904762No Hit
GGCTTATAAGATCGGGTTGCGGTTTAAATTCTTATACAAAAGCTAACACT14.761904761904762No Hit
AAGGAGAACACTCAAGCCAAAGATGTGACCAATGCGTCCACATATGTACA14.761904761904762No Hit
GTTCTAATGCCAGCTATAATTTGCAATCTTGCATAATTTCCCTTGTCATT14.761904761904762No Hit
AAGATCGAAAGGCTCTACGAGTTCTCCAGGTCCTTTGATGTTTCCTTCTA14.761904761904762No Hit
GACCTCATGAATATCCAAGCACTGGGATATTCGAAGGGGAGCCGAAGCAA14.761904761904762No Hit
ACTCTCATCATACTCATTTTTACTGGTCTGAATACTCGTCTGAACAGTGC14.761904761904762No Hit
GCATTGATGACATCGTCGGCTCTAATCAGCTTTTATAATGCTTATTAACA14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)