FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_TGA5_c24_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_TGA5_c24_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786073
Sequences flagged as poor quality0
Sequence length76
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC5750.016.43478244
CGCCGTA3651.8189894E-1216.3013698
CGGAAGG9100.015.00000170
GTCGCCG6600.014.31818245
GCCGTAT4607.2759576E-1213.6956529
GCGTCGG25950.013.352670
TCGCCGT4802.055458E-1012.3958337
CCGTATC4902.8921932E-1012.14285810
GGGCGCC2651.4063435E-411.88679244
TGGGCGC2701.6736527E-411.66666643
CGGTGGG5552.2919266E-910.7207270
CGCCGGA4259.183568E-710.70588247
CTCGGTG6007.421477E-1010.49999938
GGTGGCC2750.002545854510.18181844
GGGTCGC2900.00395186529.65517243
GTAGGGG19400.09.56185570
AGCGCGG8151.8189894E-119.44785270
TGGTCGC7051.196895E-99.43262443
TCGGGGG8555.456968E-129.41520470
GGTGGTC9101.8189894E-129.23076941
Produced by FastQC (version 0.11.5)