FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_TGA5_c24_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_TGA5_c24_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2786073
Sequences flagged as poor quality0
Sequence length76
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC5750.016.43478244
CGCCGTA3701.8189894E-1216.0810818
CGGAAGG9250.014.75675770
GTCGCCG6700.014.10447745
GCCGTAT4555.456968E-1213.8461549
GCGTCGG25950.013.352670
TCGCCGT4902.8921932E-1012.1428587
CCGTATC4953.43789E-1012.02020310
GGGCGCC2701.6736527E-411.66666644
TGGGCGC2550.001357909510.98039243
CTCGGTG5956.4028427E-1010.58823638
CGCCGGA4301.0674976E-610.58139547
CGGTGGG5451.9719664E-810.27522970
GGTGGCC2750.002545854510.18181844
TCGGGGG8650.09.71098270
GGGTCGC2900.00395186529.65517243
GTAGGGG19150.09.50391670
TGGTCGC7001.0531949E-99.543
AGCGCGG8001.2914825E-109.18750170
GGTGGTC9151.8189894E-129.18032841
Produced by FastQC (version 0.11.5)