FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_TGA5_c24_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_TGA5_c24_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCATATGTGTTATTTATTTATTGCTCTTCCCTTTCTGCTAAATTACTG11.1494252873563218No Hit
AAGGACTATCCAAGGCGTCCTTCAAGATGGCTGGAATGCCCTCGCACGCT11.1494252873563218No Hit
CCAACCTCTTTCAATATAAATGATATATCCCGCCTTAAAAAAAAAAAATT11.1494252873563218No Hit
TTGGGAGTTGTACAAAACTCCAGCCAATGTGAGTCTCTCTGTCAATTCCA11.1494252873563218No Hit
AGGAAATACTTAGGCAGAGCCCAGTTACTGGAAAAGTTCTTATGATAGTC11.1494252873563218No Hit
AACAAGGAAACACTACTTAGGCTTTTAAGATCGTGTTCCGGTGTAAGTTC11.1494252873563218No Hit
TGGTTGTACGGAAGATCCAGGGAGTTGTGCTGCTCTTCTTGGATCCATGG11.1494252873563218No Hit
GTTGGTTAAAACTTTATGACTCTATAACTTCTATACCGTGAGGCCAAACT11.1494252873563218No Hit
CCGTAAAAGGTTATACCTAGAAGAAAGAAAGATATCTCGGACAAGGACAT11.1494252873563218No Hit
AGAATTTGGTACGGTTTGACATTTTTGGAAGTTTATAATTAGTGTTGTCG11.1494252873563218No Hit
ATCATAATTCAATTTTATTTTCTGTTGTTTTTTATTAAGATATTATTAAA11.1494252873563218No Hit
CGAATCTGACTAAAAATCTCCTTTGATCTTCTGTGCTTTTCATTACTGTT11.1494252873563218No Hit
TATAAAGCTTTTGTGCATGTGTGTCAGGTTTGTGTCGTATTTCGAAGATG11.1494252873563218No Hit
AAAACGCTAAGTAGTGTTTCATTGTTAGAAGACACAAAGACAATGACTCA11.1494252873563218No Hit
CACATTTAAAGTTTACGATAAAATAACAATCATCGACAGAACTATACATC11.1494252873563218No Hit
AAAAGTATTCGGTTATTGGGGAAAAATCACTATACTTTTAATGGCGCATC11.1494252873563218No Hit
GTTGTAGTACTGGTTTTGAGGAATGAAGAAGCACCTGTTGACCCTCCTGA11.1494252873563218No Hit
CAACCTTAATATCAGCACCGCCCACAAGGGTATGCCGCTCTTCAACAGAT11.1494252873563218No Hit
CACAGATGTTGGCATTCTCAAAAGTGACTTAAATTGCTGGCGACCAGAAT11.1494252873563218No Hit
AGTCCATCTGAGTCTTTGGGTTTGTGTCTGTTAACAAGGATACAATTCTT11.1494252873563218No Hit
AGTGAAGCGTGCTGGGGCGAGAGTAGTACTAGGATGGGTGACCTCCCGGG11.1494252873563218No Hit
AAAAATCTCGGCGAGATTTTTCTTTAGAAAGTTTCATTGCTTCTAACATG11.1494252873563218No Hit
ATGGTTTCAACACCATATTGAAAGGAGGAAGTTCAACTTGCTAATATACA11.1494252873563218No Hit
TATTGATTTGGAATGTATGCTTACCTAGTGGAATCTGTAAAATGAATTTA11.1494252873563218No Hit
CGCGAAAAATACTGTACAAGGAAAAGCCAGCGGAAGATAAGGAGGTGTGC11.1494252873563218No Hit
TATTCGTGCTATGAAACACGCTTGATCTCTACGCTCTCGAAATTCAGCTC11.1494252873563218No Hit
CGCAAGGAAGAATTCGTAAGGATGGGATAATTGAGTAGTTTCGTTTCCTC11.1494252873563218No Hit
TGGAGTCGAATAGAACTTGATGTCTTGAGGATGATTGCGTATAAGCCGGG11.1494252873563218No Hit
TTCTAAGTATTTACAGATATTTGATCATTTCTCAATAATCAACTAAGTTA11.1494252873563218No Hit
ATAGCGTTACCCCTAACGGGATGAGCGGGGTGTTGTCGCCAGTGAATTTC11.1494252873563218No Hit
ATAATGTGTTTGCCTGCAAAAAGATAAGAAAATATAGGGGTTTAGGTGGT11.1494252873563218No Hit
GCTTGCTTAGGATTTGATGGACTGATTATTGAGTATTCAATGTTCTTGTA11.1494252873563218No Hit
AGCTACTTCTAGATCGTCCAATCATATACATTAGACAACTGGACGCGTAA11.1494252873563218No Hit
GTTAGAATTTAGCAGCTAGTGTGTTAAATCTAAAGAACGTTCGCCGACTG11.1494252873563218No Hit
ATCACACCTAAAAAGAACACACAAATACCAAAAAATAACATATGTAATCG11.1494252873563218No Hit
TGTCTGTTTTGCTCCAGAATGCTCCATAACCTCCAAATGCATCTCACAGA11.1494252873563218No Hit
AATCCTTTATCGAGAGGGTTATATCGAAAACGTAAGGCAACATCTCGAAA11.1494252873563218No Hit
GATTTTGTGATTACTCCGGCTTCGAGATTGAGTGACACTGAGCTGTTTTC11.1494252873563218No Hit
GCATGATTCGATTACTAACCTCGTCAAAACTAAAATATTAAAACCGTTTT11.1494252873563218No Hit
TTTTGTATGTTTCAGCACCCACTTTTAAATAATGATCCTACATTTGAGGA11.1494252873563218No Hit
GTGATCGTTCGTACCCCTCTGCTCTTCTAACCCAAGCCTGAGGCAGTCTA11.1494252873563218No Hit
TATTTTGGTGTGTCTATCCCTCCACTCACTTTTACCTAATTGCCGCTCAC11.1494252873563218No Hit
AACATCGCCCAAACGAGGGGAAGGTTTAACATACCCCCGCTAGTATGAAG11.1494252873563218No Hit
TGCCCATAAATCTCACCACAAGGAATGACAGATCGGAAGAGGGTCGTGTT11.1494252873563218No Hit
TTATATTTTTCCATAATAGGATTTATTTTTTTTATAAACTGCAGTTTTTA11.1494252873563218No Hit
AAATACAAGCAAGAAATCAAAATATTGTTTTTTCCTTATCACAACAGTGT11.1494252873563218No Hit
AAAAAAAAAAGTCATAGATGAGTCGATTTACATATAGAAACTACAAAATT11.1494252873563218No Hit
ATGCCCAAGTTATTATATATATTAGGAGTGAGCCAGATATGGAGCGAGTA11.1494252873563218No Hit
AATGAATATTAATAAAATCAAGTTTAATACTGATGTTTTCTTGCAAGTGA11.1494252873563218No Hit
CATACAGTATAAAAATATTTTGAACCTTGAACTGAAATTGGTTTAGCTAG11.1494252873563218No Hit
CGCTAGTTCTTTGTTCATAAAATTGCAGGGAACTCCGGTTAACATCATTG11.1494252873563218No Hit
TAATATGTGGAACGAGGCACTTAATAAACGACTCGGTACTGAGGTACAAT11.1494252873563218No Hit
CTTATTCGAAAGCTTTGTTGTTTTTAGGATCTGGATCCATTATTCATTCA11.1494252873563218No Hit
GAAAAAACTAGCAGCACAACGCCCTCGAGTGCGAGGCCAATTTGTTCGTA11.1494252873563218No Hit
AGGTCCTTTCCCGTGGTGAGAAATTTGAGCTTAGAGTTGACAAAACTGAG11.1494252873563218No Hit
CTTAAACCGCAGCCGGATCTTAAAGGAGTAAGAATTGTATCCTTGTTAAA11.1494252873563218No Hit
GCGACTGACTCTCGCTTGGATCATCTACACACCCTGCAAATGGATCAAGG11.1494252873563218No Hit
TTTGCAAAAAGAAAAATAGAAAAAAAGTAACTATCCGACAAATCGTGTGT11.1494252873563218No Hit
TGAATAATACATATCGAGAGTTATCTTCGTAATCAGATTCTAGCACTATA11.1494252873563218No Hit
AATGACTTATAATTTGATTATAGGTTCCGCGTTTTTGGCGATCCCCCAGC11.1494252873563218No Hit
AATTAGTCTCTTATAAAAACGATAAAATACTAATCATTATAATATCGGGA11.1494252873563218No Hit
ATAAACCCAATTTTTTATTGTTGGTTCTTTTCAATGTTACATTTGTTCGG11.1494252873563218No Hit
AATGCTAAAATTCACTACTAGAAAAATAAACAGAAACAAAGACTAGATAA11.1494252873563218No Hit
GATGTCTTCACAGTTGTCCATGATGAAGACCCGACGGACATAGAGCTTGA11.1494252873563218No Hit
CCGCATCTTATAAGCCTAAGTAGTATTTCCTTGTGAAAAGACACACAGCT11.1494252873563218No Hit
TACGTGCTTATACTCAATCATACACATGACATCAAGTCATCTTCGACTCC11.1494252873563218No Hit
ATTTTAATAAAAAAAATATTGAGATTATATACTAAATGATAGTACGATAA11.1494252873563218No Hit
TTAAGGCCCGTGGGTTGTCACATGCGTGGGAATTTCAGTTTTGCGTATGT11.1494252873563218No Hit
TATGAAATAATAGAACATTTTAGTTATCTTATTCGGATTCGTCTCTATCG11.1494252873563218No Hit
TATAATACATTAAATTGAACAAAGATTTTTAGATAGAGCTTCGAGCCTCA11.1494252873563218No Hit
AAAATTATAGACGTTGTATGGTTATCAAATGAAGCTACCTACATAGTACT11.1494252873563218No Hit
CATGAGTTCTGTTTTTAATCTCTAGCAACCCGTGCCCAAAGCTCGCTTGT11.1494252873563218No Hit
AATACTCTTGCTTCATATCATCTCTTGCCAAATTTTGTCTTGAAACCAAT11.1494252873563218No Hit
ACGAATTGAGGAATCTTCTTATTCAAGGGTTCAAAACACGCAACCTTACA11.1494252873563218No Hit
AATGAAAAAAAAAAGGTATAAACCGCCTCCCCAAACCGGGCTTGCATGTT11.1494252873563218No Hit
ACAAAGATAGTTAGTAGATCGTTAGCACCAGAGAAAAAGAGTCCTCCTAG11.1494252873563218No Hit
TTTATAGAGTAAAAATGGTCAAAATTAGTATAAATCTAATACACAAGACA11.1494252873563218No Hit
AAGCGGATGAGAGTATAGGAAGAGTTCAGTGGGGTGACGTGTTTTGTGTT11.1494252873563218No Hit
AGATTCCACATACATAAGAAGATAACTATTTAGATTGTAAGTATCCCTCA11.1494252873563218No Hit
AGTATTTTAGGTGGGACTCAAATCAGGTGTGATGCAAACAAATGAGATGT11.1494252873563218No Hit
CCGTGGGGACCCCCCTGGTTCTGTGCGGACTTGGGTCGCGATCGAGGGAG11.1494252873563218No Hit
CATAAGCAAAACCGTAGCAAAACATAGTAATGTGTTTTTTTAATGTAATA11.1494252873563218No Hit
CCACTTTGATTAACATTTGGAGCTCTTAACAAATCTCTCCTCTTCTGCCA11.1494252873563218No Hit
AGGAAGCAAGCAAAACTTTTGTTTCGTGATTCACTATTTATGATACAACG11.1494252873563218No Hit
TCTCGACCTTTAGATCGGGCGGGGGAGGTCTACTCACCCACAAGAAGGAT11.1494252873563218No Hit
AACCAAGGTCGAAAAGATTGAGAAAACTCAGTCAGTCACACCCACTGAGG11.1494252873563218No Hit
TTCCATTGCTGTACTTTTATATGGAGATGGGTACAAATAGATACGATCGC11.1494252873563218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCC50.070.061
TAGAACT50.070.011
AACGCGG50.070.070
AAACCGC50.070.051
GGATGAT50.070.029
AACCGCA50.070.052
TAAACGC50.070.068
GAGGATG50.070.027
GAAACCG50.070.050
TTTCCTA50.070.063
ATAAGCC50.070.041
TGTCTTG50.070.021
ATGATTG50.070.031
TGCGTAT50.070.036
ACTTGAT50.070.015
TCCTAAA50.070.065
AGGATGA50.070.028
TAAGCCG50.070.042
TCGAATA50.070.06
CCTAAAC50.070.066
Produced by FastQC (version 0.11.5)