FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_TGA5_7063_f-2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_TGA5_7063_f-2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633813
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG2401.0304575E-817.49994770
CGGAAGC7800.013.46149870
GACCGAG2100.00400749411.66698840
GTAGGGG3853.2734115E-610.90905870
CTCGGTG3156.9586746E-49.999969569
TCGGTGG3602.1836649E-49.72219370
TCGTGTT14550.09.14086570
ATCTCGG4006.2715175E-48.74997367
AGAGCGG4804.481421E-58.74997370
GTCGTGG17850.07.843113470
GAGTGTA14001.8189894E-127.249977669
AGTGTAG14151.05501385E-106.67842570
TCGGAAT5250.0090288626.666646570
TAGGGAA29650.06.492391668
GTAGGGA29650.06.256304767
GTGTAGA14104.5693014E-96.205654670
TGTAGGG37300.06.19301169
AGGGAAA35650.06.185114469
AGAGTGT18751.8189894E-126.159981370
GGGAAAG28000.05.999981469
Produced by FastQC (version 0.11.5)