FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_TGA5_7063_f-2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_TGA5_7063_f-2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633813
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG2401.0304575E-817.49994770
CGGAAGC7850.013.37575470
GTAGGGG3853.2734115E-610.90905870
CTCGGTG3156.9586746E-49.999969569
TCGGTGG3602.1836649E-49.72219370
TCGTGTT14650.09.0784770
AGAGCGG4753.9629198E-58.84207870
ATCTCGG4006.2715175E-48.74997367
GTCGTGG17800.07.865144370
GAGTGTA13951.0913936E-117.02506869
TCGGAAT5200.0082327216.730748770
AGTGTAG14056.2391337E-106.476848670
GTAGGGA29800.06.342262370
TAGGGAA29800.06.342262368
GTGTAGA14054.2236934E-96.22773970
TGTAGGG37300.06.19301169
AGGGAAA35550.06.1040669
GGGAAAG27900.06.021486869
AGAGTGT18751.2732926E-115.973315270
GGCGTAA8255.339773E-45.93937654
Produced by FastQC (version 0.11.5)