FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_TGA5_6911_f-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_TGA5_6911_f-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACGTGTTTGGTCGGAGCGATAGACGAGAAGAAGATGACGTGGAGCTCC20.12795905310300704No Hit
TATAAGAGTAGCCAAGAAAATGATGTGTCTATAACGCGATCTTTAGCCTA20.12795905310300704No Hit
AACAAGCACAGAGAATGAGCTAAACTTACAAAAGCAAAAGGCACACACAC20.12795905310300704No Hit
AACATTCTTCAAAACGGATTGTTCCATTCCTGACAGGAACAAATAAAAAT20.12795905310300704No Hit
ACGCTTATAAGATCCGGTTGCGGTTTAAGTTCTTATACTCAATCATACAC20.12795905310300704No Hit
TTAATTTAAAATCTTAATTATTATTTTTAACTATTTTAAGATAAAGACAA20.12795905310300704No Hit
CAACATACAAATATATGACATTTGATAAAGATTAATAGTTCTGTTAAAAT20.12795905310300704No Hit
ATACGATGACAGGTTCCAAACATTTCATAAATAAAAAGGAACATATACCA20.12795905310300704No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)