FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_TGA5_5784_f-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_TGA5_5784_f-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309645
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG2005.151869E-717.50000270
GTTCGGA1600.00909695813.12499942
AGAGCGG2254.7496162E-412.44444570
TCGTGTT9001.4551915E-108.55555570
AGTGTAG8101.0389103E-67.34567969
GAGTGTA8704.1538988E-77.241379768
AGGGAAA22250.07.235954870
GTGTAGG27100.07.10332170
GTCGTGG8502.2218846E-67.068
CGGAAGC5100.0068215536.862745370
CGTGTAG28600.06.608391369
TCGTGGA8257.781325E-56.36363669
TAGGGAA20100.06.268656768
GTAGGGA19700.06.21827467
CGTGTAT8551.2721532E-46.140351368
GTCGTGT41700.06.043165769
TGTAGGG23000.05.93478368
TCGTGTA37150.05.74697268
GTGTAGA8558.421868E-45.73099470
AGAGTGT12901.2560286E-65.697674367
Produced by FastQC (version 0.11.5)