FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_TGA5_5784_f-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_TGA5_5784_f-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309645
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG2005.151869E-717.50000270
GTTCGGA1600.00909695813.12499942
AGAGCGG2305.7150365E-412.17391370
TCGTGTT9051.6370905E-108.50828770
AGTGTAG8151.1451193E-67.30061469
AGGGAAA22100.07.28506870
GAGTGTA8805.0241033E-77.159090568
GTGTAGG27000.07.129629670
GTCGTGG8552.4363435E-66.959064568
CGTGTAG28500.06.631578469
TCGTGGA8308.4577434E-56.325300769
TAGGGAA20000.06.299999768
GTAGGGA19600.06.2567
GTCGTGT41750.06.20359369
CGTGTAT8551.2721532E-46.140351368
TGTAGGG22850.05.82056968
TCGTGTA37200.05.739247368
GTGTAGA8558.421868E-45.73099470
AGAGTGT13051.5552614E-65.632184567
CGTCGTG51000.05.62745170
Produced by FastQC (version 0.11.5)