FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_TGA5_5784_f-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_TGA5_5784_f-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAATTAAATAAAATAGGGTAATTACAATTAAGGACAATACTCCATCA15.88235294117647No Hit
ATTATTTGTCAAATGGAGTTGATTCAGCTGATTATACTGATTTGGTGATT15.88235294117647No Hit
ACTTTTAACAATGATACAATTCTTACGCATTTAAGATCCAGATGCGGTTT15.88235294117647No Hit
CCAAATCGATCATCCGATTGGCCTCCGCCGCTAGATTTGCGAAGTCCGAT15.88235294117647No Hit
GTCATCATTTCTCATCACTAAGCATGTTAAGGATAGTTTTGCATGCATGT15.88235294117647No Hit
TCTTCGTAATCCATAGAGCTTGTATCGGGTTTCCACTTTGCAGAAGAATA15.88235294117647No Hit
AAGGACAACAATGTCGAAGCAAGCATAGATGACCAGGAATTGACACTTGC15.88235294117647No Hit
AGCGGTCAAACCTTAAATAGTGCAAGACTAAACAATTTTTTAAAAAGAAA15.88235294117647No Hit
ACTCACGTTGAAGAAAGAGTCGCAGATTTCACGGAAAGAGAGAGAAAGAT15.88235294117647No Hit
GAGTATCTGATACGCGTGGCATATGTCAGCCTGTGAAATAAAACGTCGGT15.88235294117647No Hit
ACCATTTTACTTGTCTTTCATCATTTCCTTGTCCGATAACTTCCATCTCT15.88235294117647No Hit
ATTCATTTTGTGATTGGTAACACGAGTCACCATGTGGTATCATTGAATTA15.88235294117647No Hit
GTCGATTGAAGCAAAGAAGTAATTCTTTGCATTCAAATCTTTTAGCTTAC15.88235294117647No Hit
AACACCGGCTTCAAGCATGATGATTACTAACTGTTTCAGCTAAGTCAGAT15.88235294117647No Hit
AACAACTCTCGAGTACATCACCTTGCATCAGTTCTTAAGGTTTTATAGAT15.88235294117647No Hit
CGATAGGAGAGAGATGAAGCGAGACAACGTCATCTGACAAAGCTCCGGTG15.88235294117647No Hit
TCTTCCAATATGGATTATGTCACTGCTCGTAGCTTGTGGAGTTATCAACC15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)