FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_TGA5_1741_f-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_TGA5_1741_f-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1270377
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGG2001.7544768E-413.99951270
GAGTGTA8858.289135E-97.90932967
GGGAAAG16000.07.437240669
AGTGTAG8301.5279093E-67.16842567
TGTAGGG21600.07.12938170
GTAGGGA18800.06.888057770
TCAGTAA5200.0082291576.730799730
GTGTAGG23950.06.57597169
GGAAAGA21800.06.421794470
CGTGTAG25800.06.240092868
GTGTAGA8053.8956114E-46.08674568
TCGTGTT9306.1900195E-56.021295570
TAGGGAA18052.5465852E-116.010870567
GTCGTGG7750.00163416565.870762370
GAAAGAG20857.2759576E-125.70723569
TCGTGTA36950.05.683157469
AGGGAAA21251.0913936E-115.59980568
GCCTAAG14955.996062E-75.3846456
AAGAGTG15006.413611E-75.366479470
CCCGATC10453.3020612E-45.358875843