FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_MGT1_col_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_MGT1_col_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8347858
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG10400.013.79802770
AGAGCGG14000.012.49995570
CCCCTCG10000.011.89981531
CCTGGAC10400.011.77887464
TCGGTGG22750.011.692265570
CGGGGGT3104.8064117E-511.29028269
GTAGGGG11500.010.34778970
GTGGTCG16200.010.154284570
GGTGGTC16150.09.96900569
CTGGACG12400.09.59679765
TCGTGTT65400.09.25837670
CGGAAGC33650.08.9449970
CCCTCGA18800.08.93608532
CGGTGGG6704.9934897E-88.88056670
CTCGGTG23900.08.78657969
AGTCCTG17750.08.47889347
TCTCGGG19350.07.7777570
GGAACCG26450.07.674876717
AGTCCGG19850.07.58179148
CGGTGGT21400.07.523337469
Produced by FastQC (version 0.11.5)