FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_MGT1_col_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_MGT1_col_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8347858
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGG13850.013.64615170
GCGTCGG10050.013.23377370
TCGGTGG23050.012.14745270
CCTGGAC10200.011.32361664
CCCCTCG9650.011.24354143
CGGGGGT3257.773329E-510.76918369
GTGGTCG16500.09.96965370
GGTGGTC16600.09.909595569
TCGTGTT65650.09.43636470
CTCGGTG23800.09.26466669
GTAGGGG11450.09.17026570
CGGAAGC33300.09.03970
CGGTGGG6603.913192E-89.01511270
CTGGACG12450.08.71492765
TGGTCGC10051.6552804E-108.00991570
CCCTCGA18450.07.967396732
AGTCCGG20050.07.6807198
AGTCCTG17500.07.600012347
GTCCGGA20250.07.43202269
CGGTGGT21400.07.359781369
Produced by FastQC (version 0.11.5)