FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_MGT1_col_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_MGT1_col_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAACAGGGAAGATGCATTTGGGAATTGCGAGGCCTAACCTTTTGAAGCT12.1739130434782608No Hit
CGAGTTTAATCAAGTGGAGTTGCTAGAGATTCACCTAGTAGATTCAGATT12.1739130434782608No Hit
CAACAGGTTCCCGAGACAAAACAACTCAAACTCACTCAAAGTTTTAACCT12.1739130434782608No Hit
CTTATCCTAAAAGCCTAAGTAGTGTTTCCTTCTTAGAAGATACAAAGACA12.1739130434782608No Hit
TTTTGCCTCTCAAAGCTTTCATGGTGTAGCCAAAGTCCATATGAGTCTTT12.1739130434782608No Hit
CCGCAAACCAATGCCAAGAAAAAATGTCAGTTTTTGTTTTAGCATAGATC12.1739130434782608No Hit
TTAATATTTATTCAAAATTATAAAATAAATGTCATATCATCTAAAAATTA12.1739130434782608No Hit
TTGAGGATTGGCTGTAAGATTCATCGCCTAAGTGTGCATCTCTAATTGTC12.1739130434782608No Hit
CTCGCGTATTCACCAAATACCAACCAGACAGCGCTACTAGAAGTTGCGAC12.1739130434782608No Hit
TATAAACCGTGGGAATTTATCGAATGATATGCTAATCATTTCTGTATACT12.1739130434782608No Hit
ATGCAAAATGTATGATTGTATAGATTATAAATCACATCCTTCCAAAAGAC12.1739130434782608No Hit
AAAACAAAGTAGAAGCTAACGGAGCTCCGTTAATAAATTGACGAAAAGCA12.1739130434782608No Hit
TATTATGTATTTTGTATGACAAAAATCAGACTAAATAGTTTGATTTCAAA12.1739130434782608No Hit
AGACATCTCATAAACTAACTCAGGTTATCAAATCTATCTCCTTTTGCCGT12.1739130434782608No Hit
GGAGATGGTGATATAACCGTATCACACGGAAGCCTAGCAGCGAGCTTTGT12.1739130434782608No Hit
CTATCCAAAAAAATTCGAACCGAACCTAAACCAAACCTAAACTGAATCTA12.1739130434782608No Hit
TACTCAATATACTTAACCGCTAGGTAGTATATTCGAGGTCGATCCACAAG12.1739130434782608No Hit
TCTAATAAATCACGATGACAGCCCATAACATTAAATAATTGATAATTTTT12.1739130434782608No Hit
TTTCTCTGGTTTTTTGGGTTCTAAATTTGTTCCATTAGTTAGTTAGAGTT12.1739130434782608No Hit
TGGCTAATCTTGAGATAGCCGAGTAAGTACCACAACAACCACTAACCACC12.1739130434782608No Hit
AAAAAGGAATTTTGGTTCACATCCTTAGTGGGAATAATCAAACGATTCTG12.1739130434782608No Hit
GATTTCTTCTAGACATAGAAAGGGTAGAGGTCTCCAATAGATCTTCTTCA12.1739130434782608No Hit
GCTCTCCGTTGCTTCGATACAGTGTTCTTAACCACTATGCTCCTGAGTTC12.1739130434782608No Hit
TTTATCTTAGATTCCACGATCCCGATATGGATCTTATCTAATATTTCACA12.1739130434782608No Hit
GTTAAATCTTGATAGATTTAAAAAAACTACAAAAAAAATCCAGAATTTTG12.1739130434782608No Hit
TATTACTTCCGTTTCAACCCAAAAATTAAGAATTGTTAGAAATTTGAGAG12.1739130434782608No Hit
CTTTGCACTTTGAATGTCCCTGACCTGATAGTTCTTGATATATTATAGAG12.1739130434782608No Hit
CTATCCAATTGGTCAGGGAAGTTTTTCTGATGGTATGCCTCTAGGAATCT12.1739130434782608No Hit
AATATAAATGACTAGAGAGAATATTTCTGCAAGCAGGTAATCACAATACC12.1739130434782608No Hit
GATGAGGAGTCAGACTGGAGATTTTGATCACAACAGGTGGTTATTTGAGC12.1739130434782608No Hit
TATGAATCCTCCAGTGCAATCATAAGCCAGGTTGATTGTTTGTTCAGACC12.1739130434782608No Hit
ATGCAAATTTAAAGCGTATTAAGTTAGATATCCTGCATCAGACGGTGATG12.1739130434782608No Hit
AACAAAATATGGGAATATTCTGTAAGATTCATGATATTTGCTGATGGGCT12.1739130434782608No Hit
CACAAAAGTTCACAGCCAAATCATCAAAAATCAGAAAATAAAGCTTGCAT12.1739130434782608No Hit
ACTCCATCTTTATAGCATCTTCAATAATAGCTCTCTCATGTTGAAACTCA12.1739130434782608No Hit
CATCTCAAAATTACTAACTAATACAGAGTTGATGCATAATCGCACACTCA12.1739130434782608No Hit
GAGATACTAGGCTTCATGATTAAGTTCGTTTGAAAGTCAGTTAACTCATC12.1739130434782608No Hit
ATTGATACAGCAGAGAAGAATAAACTCAAGAGAGAGAGTGAAATTGAAGA12.1739130434782608No Hit
AGAATGCTGACATCATCGCCAGCTCGATAGAATATGGAACGGAAGTAATA12.1739130434782608No Hit
GAATCTAAAAATCACTAGAAAGACAGTCTTCACAAAAATATACTACTACA12.1739130434782608No Hit
AAGGTCTTTTTTTCTTTTTCTTTTTTGAAGTTTGGAAAATGAGATAACAC12.1739130434782608No Hit
CGTTGAATCCAGATAGTATTAGTAAGAGTGATCTTGAACTATGAAATAGA12.1739130434782608No Hit
CGATTCCTTGCCCGCCGGATCCGTGGTTTCGCGTATCGGCATGATCGGGA12.1739130434782608No Hit
GTCCATTACCGATCCATATCTCTCAGTTAGAGAATCTCAAGGTACTGAAT12.1739130434782608No Hit
CCTATGAGTCTTTGGCTTTGTAAGTTCTAACAAGGAAACACTACTTAGGC12.1739130434782608No Hit
TTAAAGTAATATTTGGAGCGTTGAAAATGACAATATTTTCTGCTTAGTAA12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)