FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_MGT1_c24_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_MGT1_c24_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7233138
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCC1802.8740033E-1023.33350844
CGCCGTA4900.022.14271747
CCTGGAC9650.018.85998564
GCCGTAT7000.017.99988648
GGTCGCC8300.017.71097644
CCTGGTG13650.016.9229750
GCGTCGG11450.016.20088470
GGACGAT13200.014.84849467
AGAGCGG20250.014.34568870
CTGGACG11950.014.05848865
GTCGCCG9650.013.41978945
CCCTCGA16950.013.00885832
TGGTCGC11150.012.87005143
TCCCTCG17800.012.38764831
CTGGTGG18500.012.29721951
CCTCGAC18550.012.07547933
GGTGGTC15950.011.63018241
GTGGTCG14200.011.58459342
AGTCCTG18050.011.44037147
TCGGTGG18050.011.24654470