FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_MGT1_c24_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_MGT1_c24_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAAGGACTAAAAAAGCTTGTATTAAGATATAGTTAATGATTAATT21.8181818181818181No Hit
TATGTTTCTTTTCAAAGATTTATGAAGGACTACTAATTCGAAAACACTGA10.9090909090909091No Hit
ATGGAAACTCTAACTGACCTGTTTATTTGAGAGAGTGGTCGTAGGATTAA10.9090909090909091No Hit
ACCGCAATATTCTTGTGTCGATGAGACGATGCCCGGTAACAGTTCTGTTG10.9090909090909091No Hit
AATCTGTTTCTCTAACTTATGATTACATATTACACAGCTTTATGTGCATG10.9090909090909091No Hit
AGAATTAAGGATACCCACCAAGAAGACTACTCCAATCCATAAAGATGTAC10.9090909090909091No Hit
AATATGGTACAAGACATACAAATACAGAAATGACAATGATGTCAGCATGA10.9090909090909091No Hit
AGATGCCAACGAAATACAAGAACTGCCACCCACACACATCATACACCTTT10.9090909090909091No Hit
CATACTGGGTCTGTTTTCTGTGTAGATCTGTGAACAGAGCAGTCCTAGCT10.9090909090909091No Hit
GTACTGCGCCCTGATGCAAGAACGGAGAGTTTCTGGTCTCTAAAAGAGTC10.9090909090909091No Hit
GAAGAGGAGGTGGACAACGAAGATGGTGATGGGTATGATGATTATCAGGG10.9090909090909091No Hit
GATCGTCAATTATTCAAGATGATTCTACAAGCTCTAGTGTCGTTCAAGTA10.9090909090909091No Hit
GAATCAAATTGTGGATTGTTGTGAAGTTAATCGTAGCCTACGAGTGTGTG10.9090909090909091No Hit
ACAGCTGCAAGTTCTACTTCTATCACACCGATGGAAGTAGCTGAGCCCAA10.9090909090909091No Hit
AGCAACGTACCAATAGTATCTTCAAATATTCAATATGATAATACAAGATC10.9090909090909091No Hit
TGCTAGATTCAGCTAAATCACTCGAATCTCCCCAAAGATAGATCCATACG10.9090909090909091No Hit
TGTAAGAACATTTTCAAAAGAGGTGTATACTAAGGAAATTAGTAATCAAC10.9090909090909091No Hit
AATCTCTGTCTTGTTATATTTATATCATCCCATCCAGGTAGAAAAACAAG10.9090909090909091No Hit
AGTTGTCTTCTCGAACTTGGAGGATTATCCAATCCTTAGACCTAGAAGTG10.9090909090909091No Hit
GGAATCTTCTCATCATCTAGACCTAACGAATTGGTGGGGTATATTCATAT10.9090909090909091No Hit
CTTGCTAGAAGATACAAAGCCAGAGACACCTACGGCCTTTGGCTACAGAA10.9090909090909091No Hit
TTTAGCTTTCGCACAAGCATCCCATTAAAGCTACAACATACATTCAAAAA10.9090909090909091No Hit
ACCTACAGACGCTTTACGCCCAATCATTCCGGATAACGCTTGCATCCTCT10.9090909090909091No Hit
ACCAGAGGACCGATTCCCTCTCCTGGAATCAGCGTCACTGCGCGTGGTGC10.9090909090909091No Hit
AAAGAAAACAAAACTTTAAAGAAGAAGATTTTGTATTGGAGAAAAATAAA10.9090909090909091No Hit
GAGATCTTGTGGGAAGAGTGTTGCCAAATTTATACTGTCCGCTATCTCAT10.9090909090909091No Hit
TGAATAAATAAAAAGAAAGCAATTTTCCGTATAACAAAAAAGCTCCAACT10.9090909090909091No Hit
GGATGTTGAGAAACATATAGATGCAAGAAAAAAGACGTCAACAAACAAAT10.9090909090909091No Hit
CCTTCTATGGTGGTAAGCACTAAGAAGTGAAATGTATAATATCAAGAACT10.9090909090909091No Hit
TGTAGTTGTCAATATCACCGGCCCAATCCGCATTAGAGTAAGCATGTAGT10.9090909090909091No Hit
GAGACAAGCTTCCTCACTGTCACTCCCATCTGCTTTCGCTTCAGAATCCT10.9090909090909091No Hit
CGTTGGAGATATTATTGGACCTGGATTATGCTCTCATTTATATGTTGGGA10.9090909090909091No Hit
ATTATTTCAGTCATACCCTGCCAGCATAATCTCCACATTCAACTCATTGT10.9090909090909091No Hit
CATCAACATGTAGCATTAGTAATTAAAAAGATAATGTATCCGACTATAAG10.9090909090909091No Hit
AAGTTAAGCTTTCGAGAATATATGATTCTCAAGATATCAATTTTGCGTGA10.9090909090909091No Hit
ATTATATGTAAAGAATATTCCAAATATTAATTGAAAATACATATAAAAAA10.9090909090909091No Hit
AAGCACACTTGGAGAGCGCAGTACAACGGAGAGTTGTATGCTGCGTTCGG10.9090909090909091No Hit
ATAAATATATATATGTTATGAATAAAGATGGATGCCCATTATAAGGCCCA10.9090909090909091No Hit
TGTTAGAATATTCTAACAAAAACGACATTCTAGATGATGATATTACGTTA10.9090909090909091No Hit
TCTTTGTGAAATGAAGAATCTTAGAGAATCTTAGTTACTTTGGTAAGAGT10.9090909090909091No Hit
AATCTTTTGCATGAAATCTGGATCACCGTAAGGAGCCAACATTCTACAGA10.9090909090909091No Hit
TAACATCCGCTGGGTGTTAGTAAGCAGTGATCCTCGTGGTTATCGAGAAT10.9090909090909091No Hit
AGAAATGTGTGATTTATTTATAATCTAATCTAGTTTAGTTCACAAAAGAC10.9090909090909091No Hit
TCCATACGAACCCAAGGCAAGAGCCACAGAGGAGAAAGATAATTAAGGTT10.9090909090909091No Hit
TTAATAGAAAAACTAGGAGGTTTAAAAGTTTACCTTTAACCCAGGAGCAC10.9090909090909091No Hit
TGTCTGTAAGACTCTGACTAAATAAGTTTTACTACAAAGCATATCAAAAT10.9090909090909091No Hit
TATAGAAAACGAATGTTATTGTTTTGATTCCTAAAAGATTTGCGTGAATC10.9090909090909091No Hit
ACAAGATGTAAACACAAGCAAACCGACATTTTTGAAGTATCCAAGGAATC10.9090909090909091No Hit
ATGTTGGAGAATTTTATCTTTCTCGCCATTGGAGCCACCTCAACTATTGA10.9090909090909091No Hit
ATGTTGTTTGCGCTGAAGTACATTGAACAAATCTTTGTTCAGGTATCTAC10.9090909090909091No Hit
TGGGATGATGATAACGACGGAGAACTTTTGAATCCGCCGACGTATTTTTG10.9090909090909091No Hit
TCCCCATCACCCACTCATGATCATATTTTTATGACTTAACACGCAGCATT10.9090909090909091No Hit
AGAAGCCAAGAGCCCACCATTGTTCGTGGGCTTCATTTGTACACGTCAGC10.9090909090909091No Hit
ATTAGTCCTCTCCTAAACCATTTCTTCGTTGTTATAAGGCATCGACTAAT10.9090909090909091No Hit
CATATTTCAATTATTAAGTTCCGATATGTAGCCTATTTTTTTTATAGATA10.9090909090909091No Hit
GTTTTATTAACAAAAGTGTAATTCAAAGCAATCCCAAAATCTACGGTTTG10.9090909090909091No Hit
AGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAATATCCGAAGAGCGTC10.9090909090909091No Hit
TTTAGGTATAAAATATTCAAATGCCATATACAGTCGACTCCGCAAATTAG10.9090909090909091No Hit
CGGTGAAGAGTTCATGAGTCTCTTGATTGACGGCCAAGCTGAGTTGTATA10.9090909090909091No Hit
TGTTGAGAATCCTATCTTGCTTGACTGCGCTGGGAAGTCTTTCCTTAAAG10.9090909090909091No Hit
ATACCAAAAGATTCGATTTTGCCTTTATCAGGCGAATCTTCCTTTTAAAC10.9090909090909091No Hit
ATAGTTTTTGCAAGTTCAGGATCTGAGATGCATATCCTTGGCTCTGTTCC10.9090909090909091No Hit
TGTCTTCTTACAAGGATACAATGCCTACACCTTGACGATCAAGTTGCGGT10.9090909090909091No Hit
GGTATAATATAGAGAAAAAAGGGTTGTAAGACACCCTTAGAGTTGATGGC10.9090909090909091No Hit
TATGCATTAGGATCGCGATATATGGATCTCTCGGTTTGAGATAGTAATAT10.9090909090909091No Hit
AACTGATGTTGAGAAGTCTAATGGAATCCAATATACCTTTTCATGTCAAT10.9090909090909091No Hit
ATCTGATTCGCTTGAAGAATCTCATCAGATTCAATCGCTGGGATAAATTA10.9090909090909091No Hit
AACGACAGAGATGTTAGATATAAAACCCAACTATATTGAGATCGGAAGAG10.9090909090909091No Hit
TTTAAGGAGCGACGCTGGAAAATCCCTGAATGCGAAATTATTCTTGTGTG10.9090909090909091No Hit
AACCTTCTCTTTCATTATATCATTGCCACCAACAATGAAGTAACAGACAT10.9090909090909091No Hit
AACCTCCCATTTTTATTTTTCCAGTTTGAATTTAACATATACTTCTCGGG10.9090909090909091No Hit
TACTGTAATTAAGGTTACTGATTCCAATTAAAAGAATCTTATGAGACAGA10.9090909090909091No Hit
AATACACACTTACTTTCTAAAAATGAGCATGCATTAGACATGCTTGAAGA10.9090909090909091No Hit
AAAGGAGAATCATTTCTCATCAACAAACTAGTATGCCTAGACACATAAAT10.9090909090909091No Hit
AAATTCTTTACGAAGGGGATACATTAGTTACATTTATATATGAAAAATCG10.9090909090909091No Hit
ATCGAGAGAGAATTGGATTTGGTTAGACAATGTGTGGTTGGTAAACACGG10.9090909090909091No Hit
AGAACTGGCGATGCGGGATGAACCGGAAGCCGGGTTACGGTGCCCAACTG10.9090909090909091No Hit
ATATGTACAGTTTGAAACAAACTATTTAAAAATGAATTTCAGACTATTTT10.9090909090909091No Hit
GTCCGGATCGACGATTCGTCGGACCCGGTAAACTTTTCTCGACGATAACA10.9090909090909091No Hit
AAAAGTCAATGGAAAAGTTTCATTGTCTTCCTTCTTTCGTCGGATGGCTC10.9090909090909091No Hit
TAATCCTGTTTTAAAGTAGAGAAGAAGATAAGAGAAGAGAATAGACAAGG10.9090909090909091No Hit
GGTTACTGTTCATGAAGAAATGCAAAGCCATAATCTAACATTTATTGAAC10.9090909090909091No Hit
CAGATTTGGAGGGTGTTGCTACCATGGCTGGCAGAATAGCCACAAAGAGA10.9090909090909091No Hit
AAGGTATAATAGAATATACCATAAGATTCCGGGGGAAAATTCCGATGAGA10.9090909090909091No Hit
TTTATATAAAATAAAGCTATCCTAATTTTTAAATCAAAATATCTAAATCC10.9090909090909091No Hit
AAATCCTTGCCAGATTCACATTGCAGTTACCGGAAGAAGTACGAAGGCAA10.9090909090909091No Hit
AAAAGAATATTCTTAAATATATTCTATCTCTAGTTTATTATTAATTATGA10.9090909090909091No Hit
CTTATCCTTGCGATGGGCTGAGACTAGGTTGCGCAGTTGGTGACAATCAC10.9090909090909091No Hit
GCTCGCATTGGTGAATGAGTTGCGGGTTGAAATCTCGGATGCGGAAAAGT10.9090909090909091No Hit
ATTTGGGAACACGACAATAGTTGATGAGATATCAGAATTCGGTTAGGTAA10.9090909090909091No Hit
AATTCCATCGAATTGAGTATGATTGTATGTGTGATAGCATCTACTATACC10.9090909090909091No Hit
GGTCAATCACCTGCTCCAAAAGACCCCTTTGAATCTTGGATACAACAAGA10.9090909090909091No Hit
CAAAACACTAACCAACCTTCTTCTTGCTTATCAAAGCTTTCATGGTGTAG10.9090909090909091No Hit
AGATATTTGGACTAGTAGTCAATTCACATGATGTGTACAACCAGTGGAAA10.9090909090909091No Hit
ATGAAGACTCAAAATTTCTCCCCCAAAAAATAAAATAAAATGAAGACACA10.9090909090909091No Hit
GCAGCCAAACAGATAAAAGAGAGGTATGCCATTACACTCTCTCTAGAGAA10.9090909090909091No Hit
CTAATCGTGTAGGCGATTTTCGTTTAATAGTAGGAATTTTAGGTCTTTAT10.9090909090909091No Hit
CTGCAATCTCAAAGCCAACTTCTTCATCATCTTCATATACGTACTTAGGA10.9090909090909091No Hit
AATAATTAGATGTCAGTAAATAGATTTTTATATTACAGAAAAAGAAGTCC10.9090909090909091No Hit
TATAATATTGACAATTTAGAAACCTTACAAGAAAACAAACTATCTTTATT10.9090909090909091No Hit
AGACATACCAAGCCCACTATAAGTTTTTAGTGATGCCTTTCGACTTAACT10.9090909090909091No Hit
TCAACTGTAGGCAACTGAAGTAGCATCTCATCCATGATACCCATCAAATG10.9090909090909091No Hit
AATAGTTTTAAGCCTTCAAAACCAAAAACAAATAAATACGTTGTCGACAT10.9090909090909091No Hit
AGTAGTCTTTACACATAAGAAACAAAAAACAAGAAGTGATAGAACCATTT10.9090909090909091No Hit
TACGTTCAATCTTCGACAGAGGTAAGCGCAGAACTTCACCAATGTAATAT10.9090909090909091No Hit
CCTGATCTCGAGTTGCCACAAGATGCGCCAGATCATCCTGAAAGTTTAAC10.9090909090909091No Hit
CCCAACACCCTTTGTGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAAC10.9090909090909091No Hit
ATATGATACCACTAAAGAATGAAAGTATGTATACAGGGAAAATATTGTTT10.9090909090909091No Hit
TATTAGTAAGAGAGGTGTCTTAGGCAACCAAAGATGATCAACCAGAATAG10.9090909090909091No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers