FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_LHY1_col_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_LHY1_col_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8730615
Sequences flagged as poor quality0
Sequence length76
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG6950.013.0935370
AGAGCGG13550.012.91513370
GTAGGGC5251.2573764E-710.00000370
CGGAAGC19950.08.77193370
GTAGGGG9901.2732926E-118.48485270
GCGTCGA5450.0016614837.064222368
GAGCGTG7956.1920127E-67.044027370
TCGTGTT42350.06.446283370
GTAGGGA68850.06.100219769
CGTGGAG32350.05.51777670
TAGGGAA78800.05.50761670
TCGGTGG11503.937346E-55.47826370
CTCGGTG9050.00173433025.414366769
GGGAAAG70250.05.281140368
CGTGTAG109650.05.2667670
AAGAGTG65850.05.261960570
GTGTAGG99900.05.25525770
TGTAGGG91600.05.23471868
AGAGTGT51150.05.200392770
GTCGTGG46750.05.09091170
Produced by FastQC (version 0.11.5)