FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_LHY1_col_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_LHY1_col_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8730615
Sequences flagged as poor quality0
Sequence length76
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG6900.013.1884170
AGAGCGG13600.012.86765170
GTAGGGC5251.2573764E-710.00000370
GTAGGGG9750.09.33333670
CGGAAGC20250.08.1234670
GAGCGTG7904.2813324E-56.645571770
GCGTCGA5300.0099018666.60377668
TCGTGTT42050.06.49227370
GTAGGGA69450.06.09791469
TCGGTGG11355.1330135E-65.859032670
TAGGGAA79150.05.439041670
CTCGGTG9050.00173432995.414366769
GGGAAAG69900.05.407727268
CGTGGAG32100.05.34268170
CGTGTAG110550.05.31886270
TGTAGGG92150.05.279437568
AGAGTGT51100.05.273974470
GTGTAGG100250.05.271822570
AGGGAAA98500.05.25888570
AAGAGTG65550.05.125859770
Produced by FastQC (version 0.11.5)