FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_LHY1_c24_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_LHY1_c24_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4951402
Sequences flagged as poor quality0
Sequence length76
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG35200.06.46299370
GTAGGGA16501.6370905E-116.36362769
GCGTCGT37150.06.312238769
GTCGTGG7802.5983062E-46.281978670
TGTAGGG19550.06.265975568
GTCGTGT29800.05.98992469
GTGTAGG20500.05.975600767
AGCGTCG40300.05.90569968
CGTGTAG23300.05.85830170
GAGCGTC44350.05.76092670
CGGAAGA78700.05.736967669
AAGAGCG58500.05.62386770
GGAAGAG77050.05.587216470
TCGGAAG81750.05.39448868
TCGTGTA33850.05.376653769
TCGTGTT13804.3597956E-65.326025570
AGAGCGT49950.05.325263570
GAAGAGC67950.05.15078770