FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n02_LHY1_c24_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n02_LHY1_c24_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length76
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTTCTTTATCAATAGATCAAAAAAAGCAAACACATTGATTTGGTGGC11.7241379310344827No Hit
AAAGCGGGGTTTTTGTTGTAAGATATCTAAGGACACCGTCGCTGGAATTG11.7241379310344827No Hit
GATTAAGAGTTGAGCACTGTAAGACTATGCATATATCCAGTTACTAAAGA11.7241379310344827No Hit
TGAGAGAACATTTTTAAAATATTTTTTAAAGTGAAGACATATATTTACAA11.7241379310344827No Hit
AAATCCGCAACAAGAGAAAATAGTTGTAACTTTTATATTCAAGATAACAT11.7241379310344827No Hit
ATAAACTATACTTGCCTTTAGTTATGAAGATTATTATAGAATCAATTTTT11.7241379310344827No Hit
TCCCGTACAAAAACCAGTTATTTATATTAAGGTTGATCTTAGTTATGATA11.7241379310344827No Hit
ACCTTTGCCGATCGACACAAAAAATAAGCTAGTTTGTTTAAATATTCTCA11.7241379310344827No Hit
TTCAAAGAACAGATCTTTGCGAGACTTTCAAAGAACAACAGAATTATTCG11.7241379310344827No Hit
ATTGAAGGTAACAAATTTATGACACTAAAGTTTGATATTTTGGTTTGTTC11.7241379310344827No Hit
AAGAAGAAGCTTGGATAGTTTTTTGTAGTCAAATATGACTAGAAGTCAAG11.7241379310344827No Hit
TATTTCTTATAGCATTAGGGTTTAATATCTTTCTTTTTTTTGGACAAAGG11.7241379310344827No Hit
TCTCCATTTCGTATTCTAAGAATATCCTTTCCGCTTCGATGTGCTCTAAC11.7241379310344827No Hit
CATCCCCGTCTTCCCAAATCTGACAGATACAGAGATACTTAACTTGGTCC11.7241379310344827No Hit
TATTGCCTTTATATTAATATTTTCTATTTCAATTTAAATCTTCTTCCATA11.7241379310344827No Hit
TATTTTAAGTTAGTATATTTTGTATATGTATTGTATTATTTGTATATTTT11.7241379310344827No Hit
CAAAATTTGGAAATCTGATATATATACCACTAAAACCTTCTCGAGACACC11.7241379310344827No Hit
ATCAGGGGTCAAGTCATCGACAGAGTTCACAGCTACTCCACAACCTGCCA11.7241379310344827No Hit
TCAACCTACACGAACATAATCATAATCTTTTGTTTCAAGTTGGTAGGTGG11.7241379310344827No Hit
CTGAAATCTAAAGATATCTTAGAATATCTTGTATTTTTATTATTTCTATT11.7241379310344827No Hit
TACTATATAAAAGTAAGGGAGATAAAATGTATGTATCAAACTGGGACTAG11.7241379310344827No Hit
CGGGCAAAGCAATTCCTCTGCCAATGGCAAAATCGCTTTTATGAACTTCC11.7241379310344827No Hit
CAGAGTTGGCTAGTAATGTTTACGTCGGATAACAATCTAATGAAAGGAAA11.7241379310344827No Hit
CGGGAATCTCATTATGTATTCGGTGTGGATAAAAGATCTTCCTATGTTAT11.7241379310344827No Hit
AAGGCCTTGCTACAAGGAGAGAACATTTCCTGATGAAATTGCTTCCCAAA11.7241379310344827No Hit
TGCTTTAAGTACTATAATATCTAATTATAATCATCTACAGTGAAATTTCT11.7241379310344827No Hit
TACTAAAAAATCCAAACAACAGAGACTCAATTGCATACCAAAAGATATTT11.7241379310344827No Hit
ACATATGTAGTCTTTCTTTGAAAAGTTATAAATGTAAATGCTAGTCAAAG11.7241379310344827No Hit
TTAGTATATAATTATCATTTAAGATCTCGGTATTGTGCTTTTTTCTATTT11.7241379310344827No Hit
GCAATTCTTAGGATATTTGCATCAGAATTACGGTTCAGGAGTTCGAGATC11.7241379310344827No Hit
TTTTATATCCAGGTTGATCGTACAAAACTAAGACCTATCGCCAGTGGTCT11.7241379310344827No Hit
TGATACCTCCTTATATCAGAGTTCAATACAATCATAATCTTATTAAAACT11.7241379310344827No Hit
TTTTTAGATTATGCAACTCGAAAAAATCCACATAGAGGATCGTTTAGAAA11.7241379310344827No Hit
CAAATCAAAATCGTTTTTCCAATTTTCTAATGTGCCATGCTTTCTGATAG11.7241379310344827No Hit
CAAGAAAAAAAATCATATAAATAAATTCACAAACTTACACATTTTCTTCA11.7241379310344827No Hit
ATTAGTTTCCTCTGTTCGATCGGCCATTTGTTTTTATCCATACCCGTTCT11.7241379310344827No Hit
TTTGAAAATATCTTCTGTTTTTTTTATAGCGATTAATTTAGATAAAATAA11.7241379310344827No Hit
TCTAGTAATGATATTTAGTCAATTTGAAATATCTTTTATTGGTTTTACTT11.7241379310344827No Hit
TAATACAAAATCTTTTAAGGTATGATTTTCTTTCCATATTTATTTAATAT11.7241379310344827No Hit
GAAGTAGGTCAAAACAAAATCAATAACAAAATATCTGATAGCGAGAAGCT11.7241379310344827No Hit
ATCAGGCAAGAATGATCAATATCCTCATCTCGATATAAGGTGCCCCCCTA11.7241379310344827No Hit
TCCAATAACAGCCCCTACAGAATTTTTACCAGAATGTTTAGGGATTGTTA11.7241379310344827No Hit
TATCTTGGATTTTGTTATTATTTCTTAAAACGAATTAAATCAATAAAATA11.7241379310344827No Hit
GATAGATATTAAATTGTTGACAGCTATATTTAAACTAATCAAAGATTGTA11.7241379310344827No Hit
CAACTAGAATAATCACTTGGAACACTCAGAATATTGCAGCGGGCCAGTTC11.7241379310344827No Hit
TGTCAAATCTGTCCCGCCAACCCGTCTCGTAAAAAAAACAAATATATGTT11.7241379310344827No Hit
TGATTTTTTGGAAAACTTCAGAAGATAAACTTTTGAAATCCAAATAGTTT11.7241379310344827No Hit
TTAAGAAACTATACTAATAATTTTAAAAACATTTACAAAGATAAATACAC11.7241379310344827No Hit
AAAGCAAATAGAGTTTTCCTAGAAACCAACTCAGACACATAACCTAATTT11.7241379310344827No Hit
AACCATTTGACGACAGACTCCGCAAGACCATTTTCCGGATGGAAGAGAGG11.7241379310344827No Hit
TTATATAAACCAAGATTGTTAATATATTGTTCAAACAATCTAGAACAAAA11.7241379310344827No Hit
TTCCGTGAGGGATATTGGTAAGGAATTCGAATGTAATAATATCGATTCAT11.7241379310344827No Hit
ATTATGAAAATGAAAGATCCAATTTGAAAATTATATCTTATAGATATAAA11.7241379310344827No Hit
TTTATATAAAATATTTTATTTTTAAATATACATAATACTTTTGATTATCA11.7241379310344827No Hit
GAGTCTATCACTGAGCGGATATTCTGTTTTGCAGCGGTTACTAAGTATCA11.7241379310344827No Hit
AGAGTTAACCCTGAAATCATTTTTATTTTGTGTTTCCATTCATATACTAT11.7241379310344827No Hit
ATAGATATTTACTTTTTGCTCTAAGTCTTTATATATAATTTCATATTCGG11.7241379310344827No Hit
TTTATATCTGCAATTTCTTTTTTGTATTAAACCGCAATTGTATTTTCATG11.7241379310344827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGT50.070.027
TTGTTTC50.070.030
CTACACG50.070.06
CTACACA50.070.067
AATCTTT50.070.024
ACACGAA50.070.08
TCAAGTT50.070.035
ATCTTTT50.070.025
CCTACAC50.070.05
CACACAT50.070.070
TGGTAGG50.070.041
AATCATA50.070.018
TTCAAGT50.070.034
CAACCTA50.070.02
GTCAACT50.070.062
CAACTAC50.070.064
TAAAAAA50.070.053
TGTTTCA50.070.031
GGATTAA50.070.049
TTTCAAG50.070.033
Produced by FastQC (version 0.11.5)