FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30896798
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG730760.2365164183032818No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT43450.018.0509226
AGGGGGG57500.015.7137911
TATGCCG53650.015.4672133
TGGGGGG181950.015.205621
ATGCCGT63800.013.0616534
GTATGCC65200.013.0496172
TGCCGTC64650.012.998245
CGTATGC69000.012.8410491
CCAGTCC82650.010.62993770
CCGTCTT78850.010.3406027
GAGCACC138500.09.32559270
TACGGCG23100.09.0945346
CACACGG55900.08.766294570
CTGCTTG81700.08.65196114
GCCCCCT25600.08.3448581
ATCGGGG43050.07.88556942
CGTCTTC105850.07.83518178
GGCCCCA23600.07.71650081
AAGAGCC182550.07.57382270
ACAGTTC84450.07.50092532
Produced by FastQC (version 0.11.5)