FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_pIXHALO_col_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_pIXHALO_col_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2896455
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTCC5100.015.78439170
GAGCACC8450.013.25450370
CCTTGTT27050.012.42150370
CCCGATC14050.011.95735442
GCCTAAG25000.011.62005755
CCGATCT15200.011.28294944
AGGCGTA8750.011.20005453
CTACCCG3155.6548106E-511.11097333
ACCCGAT14250.011.05268742
CCTAAGT25600.010.80083556
CAACCCG13700.010.729979539
AAACCGC31400.010.70050633
AGCCTAA27750.010.4685254
AACCGCA33250.010.421103534
ACCGCAA29400.010.35719435
AAGCCTA28200.010.17735553
TCCTTGT33300.010.09013969
GTAGTGT24300.09.9383261
CGCCAAT20600.09.85441867
AAGAGCC12450.09.83940670
Produced by FastQC (version 0.11.5)