FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_pIXHALO_col_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_pIXHALO_col_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2896455
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTCC4900.015.71433970
GAGCACC8400.013.33337970
CCTTGTT27100.012.78602170
CCCGATC14200.012.32398642
GCCTAAG25000.011.76004155
AGGCGTA8800.011.53413153
CAACCCG13900.011.07917539
AAACCGC31500.010.99984833
CTACCCG3206.6375345E-510.93734833
ACCCGAT14450.010.89969241
CCGATCT15450.010.87382444
CCTAAGT25800.010.85275156
ACCGCAA29550.010.778378535
AACCGCA33350.010.70468534
AGCCTAA27900.010.5376754
GTAGTGT24200.010.413259561
AAGCCTA28300.010.26505353
TCCTTGT33450.010.25414669
TAAACCG36850.09.97272532
TAAGTAG28450.09.96488658
Produced by FastQC (version 0.11.5)