FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_pIXHALO_col_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_pIXHALO_col_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACCATCTGAGTGCCGTGGCGCAAAAGGACATGAGAGAGAGTGAGAGA11.4285714285714286No Hit
AGGGAGTCCTTAGCAAAAAAAATTGAAAAATAAATCTGTTAACCTTTACA11.4285714285714286No Hit
TAAGACCTTGAGTCCAAGTAAAGGAATAAGGATTAGCGAATCGGCTTTCT11.4285714285714286No Hit
CTGCTGAGATTTGCGTGATGTCATTCCCCAAAAGATTTTTAGGCAAGAAC11.4285714285714286No Hit
CCTTGTGAAGGTGCTGCAATAGAATCTGGAAAGAATCTTCCTCCGGATTA11.4285714285714286No Hit
GGCAATGTTGTTGATGACATCATATGTGAAACCAGGTGAGATTGAAGTGA11.4285714285714286No Hit
AAACTAGTCTCAAAAGCAAGAAATGTCATAAGGCCAAGATTCCACGCAGC11.4285714285714286No Hit
GTGATGTAAGCGACATGGATGCAGCATAGTTAGCAGATGGAGAACATATG11.4285714285714286No Hit
TATCTTTTTGTCTTTTTCTATTTTTGGTGACGCTTGACTTCACGGATCAT11.4285714285714286No Hit
TTCTCTTTTAAGATTCTATATGACGTCATTTCCCTTCCAAATGACATTTG11.4285714285714286No Hit
CCTCGTGTTTAGTTGCCACCGTTGAGTTTGGAACCCTGAACAGACTGCCG11.4285714285714286No Hit
TAGCCTCTATGTCCTGAATTTGTTACGTGTAATTAGACTACCGGAAGGAA11.4285714285714286No Hit
ATGTCAATGTGACGTCATAAAAATGCCACTCTTTAGATCTTTGATTATTT11.4285714285714286No Hit
ATTGAGCGGTTATGAAATTGCTTTGCCCCATATTAGGCTGTCTTGCTACA11.4285714285714286No Hit
TCAGAAATACTGTAATGAAAGGAACAGAGGAGTTTGTCGCTAGGTATTTG11.4285714285714286No Hit
ATGTAATTTTGTTGATGAAGAAACAGCCCGTGAACATCACGCTGAACTGA11.4285714285714286No Hit
GTGACATCAAGTCATATTCGACTCCACACCACTACCCAACTTTCTTCTTG11.4285714285714286No Hit
CTAATATGAGTGTCAAAAAATACAAAATTCGATGGTAGCGATGAGCAATC11.4285714285714286No Hit
AAATAATTCTAATATGGAAATTCTAAATTGTTGACAAGAAAAACAAAAAC11.4285714285714286No Hit
CCTATGAGTGACGTAATACATGAGGTGGACACATATTATTATATACACAT11.4285714285714286No Hit
TATTTGGCGCCATTAGATCAAAATATGCGTTGATATATCAGATTTAAACA11.4285714285714286No Hit
GGCCACCACCACATGTCCCTTTTCATTCACAGAAATTATTTTGTTGCTAA11.4285714285714286No Hit
AATTCATACCTAAACATTTTCTATGTGCTAATACATACCCAAGTTCTGAA11.4285714285714286No Hit
GTAAGAAGGACAATAAAGTTGAATGTAAGAGTAGCAAAGGCGCGACTTTG11.4285714285714286No Hit
GATGGCGGCGATGCCGTAATCCTCTGTTTCGATGAAGGATACTGGAGATC11.4285714285714286No Hit
TATTTTATGAAACTGAAATTGCAAGGGTTTCAACCTCTTTCATTTCTCTT11.4285714285714286No Hit
TAGGTTTGTTGCATATGCATAGGGGATGATGATGAGATAGCCTTGTAAGC11.4285714285714286No Hit
CAAGACAAAACGTTGATCCGCAACAAATGTGGACAGCCGATGGAATTCAA11.4285714285714286No Hit
AGATATGTTGATGAATTGGTGATAGCATAAGTCATCTCAAGGCAATATGC11.4285714285714286No Hit
CGCAATTTTGACATTCGTTCACAATTTTGCAACGTCATCAAGAGAATATT11.4285714285714286No Hit
AGATTCAAGGTCAATCATATGTTCATCTTGAATTAAGATTAAGACAAGTG11.4285714285714286No Hit
AAGTTGGTCACCTTGCCTACGTCATCCAAAAGAAGGTTAGGGGACAATGG11.4285714285714286No Hit
GGAAGCCTATCTGCTCAGTCTACACTCAACCAGACAAGCTGAGAACAAAG11.4285714285714286No Hit
TCGTGTTTAGTTGCCACCGTTGAGTTTGGAACCCTGAACAGACTGCCGGT11.4285714285714286No Hit
ACCGAATGGCGGTGTATGCTTATATCCTTAAGCTGGTTTATAGGATTCAC11.4285714285714286No Hit
TGACATTATTAATGAAGTTTGCGATTTTCATGTACCCATGCAAGATCGGA11.4285714285714286No Hit
GTTCTAAAATCTGGCAGGACTCTCTCACCCTCCTAGTCCTTCCCAAACAA11.4285714285714286No Hit
CAACATAGTTATAGATGGTGTTATTGTCAACCTTCATGAATTGTCTCATG11.4285714285714286No Hit
TGGTGCATGGCTGTCGTCAGCTCGGGCCGTAAGGTGTTGGGGTAAGTCCC11.4285714285714286No Hit
GCAAAAAACAGAACTGATTGATGACGTCTTTTGTAGTCAATACTTATGAT11.4285714285714286No Hit
TATTATACTGAAAACATCATAACTTATAAATCGAGATAGTGATGATGATG11.4285714285714286No Hit
AGATAACGTCAGCAATGAATATTAGCCTGTTTTAGGACTTTAGAGTCCGA11.4285714285714286No Hit
ATTTCCGAGATCTTCCCCTAGCTCACTGAAATTGATTCCTGTTATCTCTA11.4285714285714286No Hit
TTCCATTTGCTGTCGGAATTCGCCAACTGGATACGGAGCTGCCGTAAGCG11.4285714285714286No Hit
AATTAAATAATATAGTTCGATACACGAATATAATTGCGAAGAATAGGCAT11.4285714285714286No Hit
TAGGCCTGAGGATACGTCCCAATTCTCACCCTCCAAACCATACATATAGT11.4285714285714286No Hit
GATGACGAAATGGCAAGAAACACGTCGAAATTATTGACCCAAATTAATCG11.4285714285714286No Hit
TTCATCATAACATTCTCTCCCGCCGCTTTCTTTCATCTTTAAACAAACGA11.4285714285714286No Hit
TTATATATCTCTTAATATTTATTTTTGAAAGGTCGTCATCACTTGATTGC11.4285714285714286No Hit
GTTCTAAGGGTAGTTTCGTCATTGTGATAACACTCTCCATCCGGTATGAC11.4285714285714286No Hit
ATTTGGTACGGTAAGCAACCAGTTAACTTGTTATTCAAGAAAAGGACTTC11.4285714285714286No Hit
GTATGTTTTGTGGAATCCTCAGCCGGAAGAATAAAAAGCCTTCGTCGTCT11.4285714285714286No Hit
CGTAACATTACTCACCTCTATAACATTACTCAACCTTAATAATATACGTC11.4285714285714286No Hit
CCGCGCAGCGGGATGATTGACATCAGCACCAATGAACATGACCTGATCCT11.4285714285714286No Hit
CAATCGCTGCCCCACCTACTCTAAGTGATGCGGTTAGACCTGATACTCTG11.4285714285714286No Hit
CATGAGACTTTCATCTCGCACGGCTCCTAAGTGATAAAAGTAAAGAAGAA11.4285714285714286No Hit
CATATGTACTATAAAAGGGTAATGATCATATTGTTATATCAGGAGGAGTT11.4285714285714286No Hit
CTTGTAGCCTCCGCTCGTAAGACCTAAGAAGCAAGGTGTGTGCGCGAGAA11.4285714285714286No Hit
CCTGCCGTCATCATATCTGCGTGTTGTGTGTCAGCATGGCCACGTCAGGG11.4285714285714286No Hit
TCTTCTTCTTCTTATCCTCATCATCATGATACTCCTTCTTCTTCTTCTTC11.4285714285714286No Hit
AAGCCTGAGGTAGAAAACAGAGGAGGAATCTGCAGAGTGATTGTTTGATA11.4285714285714286No Hit
TGAAGTATCATCTTGCTTTTACTAATGGTGACATAAGGATTTTCAGTCCT11.4285714285714286No Hit
ATATATGAGGAGAGTGCGTTAATCTATAGAGAGCAGATGAGCGGAGGTTG11.4285714285714286No Hit
TCTTAAGAATTGGAGATTGGACATTCCTTCAAAAGCTTTTTCACTTATAT11.4285714285714286No Hit
CTTCTCGCTTACGTCACTTTCCACAGATCCAACAATGATATCAGATAAAA11.4285714285714286No Hit
GCCGCAGGGGCAGGAACAACGCATCTCTTTATGATAAACGGATCCATGTT11.4285714285714286No Hit
TGCAGCCTGCAATCCGAACTGAGGACGGGTTTTTGGAGTTAGCTCACCCT11.4285714285714286No Hit
AAGGGTTTTCGTTTTTATCTTTTCCGACGAAGAAGAAGATAAGAGGTTGA11.4285714285714286No Hit
CAATTTACTCAAAACTCGTACCAACATAACATATTGTTGTTACCAAATCG11.4285714285714286No Hit
GCTATTATACTCACCAAGCCCACCTTTCACACCCTTCTACGATTGTGATG11.4285714285714286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTTAT50.070.028
CAAGAAA50.070.036
CCAATGC50.070.070
GTCACCA50.070.066
CAGTTAA50.070.020
TCAAGAA50.070.035
ACTTGTT50.070.026
CCAGTTA50.070.019
AGGACTT50.070.043
TTGTAGG50.070.051
TGGTACG50.070.04
GGACTTG50.070.057
GGACTTC50.070.044
ACTTGAT50.070.059
TTCAAGA50.070.034
TACGGTA50.070.07
TAAGCAA50.070.012
CAACCAG50.070.016
GAAAAGG50.070.039
TTATTCA50.070.031
Produced by FastQC (version 0.11.5)