FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_pIXHALO_c24_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_pIXHALO_c24_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5368317
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTGTT61950.013.22035770
CAACCCG24150.012.89844739
AACCGCA65150.012.57088934
ACCGCAA60200.012.55803935
AAACCGC65050.012.5364133
CCCGATC26800.012.14565242
GCCTAAG51250.012.08793355
TCCTTGT69650.011.95970469
GAGCACC14550.011.78695770
CCTAAGT53550.011.6995856
CGCAACC49400.011.40679237
GTAGTGT48450.011.26957661
CCGATCT30000.011.08345243
CTACCCG6401.8189894E-1110.93741333
TAAACCG75400.010.81556432
ACCCGAT28200.010.79778641
AACCCGA28900.010.778460540
CCGCAAC60000.010.73324836
AGGCGTA24600.010.67074653
GCCCCCT2300.00789442910.65228751
Produced by FastQC (version 0.11.5)