FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_pIXHALO_c24_b-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_pIXHALO_c24_b-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5368317
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTGTT61500.013.31709170
CAACCCG24050.012.9520839
ACCGCAA60450.012.44820535
AACCGCA65350.012.425302534
AAACCGC65250.012.39070633
GCCCCCT2305.7196215E-412.1740431
GCCTAAG51500.012.09721655
CCCGATC26650.012.08268142
TCCTTGT69600.012.01858169
CCTAAGT53950.011.67771256
GAGCACC14550.011.54640770
CGCAACC49300.011.42992937
GTAGTGT48500.011.257956561
CTACCCG6301.2732926E-1111.11102233
CCAGTCC8850.011.07346270
CCGATCT29850.011.02189343
CGGGGTG2250.006705960710.88890452
AACCCGA28700.010.85357240
ACCCGAT28050.010.73075341
TAAACCG75400.010.72272732
Produced by FastQC (version 0.11.5)