FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_pIXHALO_c24_b-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_pIXHALO_c24_b-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTTATGGCTCATCCCCGGAAATAACGGCAACTGCGAGCCAAAAACTCA11.9230769230769231No Hit
GAAGACGTAGATACTTGTAGAATACAAGAAAAGAAATATCCTCTGTGGAA11.9230769230769231No Hit
CACCCCGTTTTGATGATGTCAGCAGTCATATCGGCGATAACATGGACGGC11.9230769230769231No Hit
AGCTCTCGATTAAGCGTGAACTTGGAGGATCAATGGCTGACGTTCGCACA11.9230769230769231No Hit
GATGTCATGTGTATGATTGAGTATTAGAACTTAAACCGCAACCCGATATT11.9230769230769231No Hit
GTAATAGCTATGAAATGTGAAAAGCTGAAGGATACGTACCTTCAGCACAT11.9230769230769231No Hit
GCCGTGTCTACAGGTGGCTCACAGGGAGGCCTTCAAGAAACCCTGTTATG11.9230769230769231No Hit
CGGCAGGTTGCACTACTTCCTTTTTACCACCCGTTTTTAGCTTTTCCTCA11.9230769230769231No Hit
AGTTACTTTTTGTAGTTTTCTTTTTTTTTTTTCAATTTTGATGAAATACT11.9230769230769231No Hit
GCGCAGGCTGTGACATGTTTAGCTGCGAAATGGCATGTATGAGCCACGTT11.9230769230769231No Hit
CACGTGCGGGGAAACCGTCTTGTATTTAACCGATGGTAGATCTACGTAGT11.9230769230769231No Hit
TCCACATGTTGGTTAGAACCTTCTAGAATCAGAAACAGTAGCTAGGGTGT11.9230769230769231No Hit
CTTTGAATTCTCTTCTTCTTCTCCTTCCACTACCTCTTCTTCCTCATCAT11.9230769230769231No Hit
GCCCACATTAACCGTCGAGCCAGGAACCGTGTTCATGATCCCAAATGCTA11.9230769230769231No Hit
ACTTGCCGTATAAGTGGAATCGATTTTTTGAAAGCATACATGGGGTTGGG11.9230769230769231No Hit
TCTATATATAACGTAAGCATGTAAAAAGTTATGACATAATTTAATTGAGA11.9230769230769231No Hit
TGATTGTCATAAGAAAAATGCAGCATGATCTAATTTAAAATTTTTGGATC11.9230769230769231No Hit
CATATAGTTGCATCACATTTAGGATAGAGTCTACGATTTTTCATATAGAA11.9230769230769231No Hit
AGATGTTTTATGTCAGTTAAGTGTCTTACGTAAGTAAAGTTTGTATTAGG11.9230769230769231No Hit
GTGTCATGCTGCTTTTAATCAGATTAAGAATGCCTTAGTGAGTGCTCCAA11.9230769230769231No Hit
ATTATTTTATTATTTTTGAGTGGTGATCCTTTTTCCATATGACGTATATA11.9230769230769231No Hit
GTCAGGTGGTGATTCAGCGTCCGAGATGGAATCGTGATCGAGTGAAGCGA11.9230769230769231No Hit
TTCCGGTTAGTCTCCTCAATGATCTTCTGGTTAACTGCTTCGGCGCTTGG11.9230769230769231No Hit
TTACATATCAGACCTATTTCATTCTGTTTGGACCATGTAGTTATTTATCC11.9230769230769231No Hit
TATAATTTCATTTGTAGTGAAACCGGAAAGATTCGTGAAAGTAAAAATTA11.9230769230769231No Hit
ATGACGTAGTTATAATACAATAGAAATATAATTTTCTATAGTTTCATAGA11.9230769230769231No Hit
ATTCTCATGTAATGAAGTCAGTCATGTGTGATGTGTTTTTCTGATGACGC11.9230769230769231No Hit
CCTCTAGCTTCAAATTCCGAAGGTCTAAAGGCTCGATAGGCCACGCTTTC11.9230769230769231No Hit
TAACGATGTTAAGAGCCATGCTTCTTTTGCTTTCAATGACTATTACCAGA11.9230769230769231No Hit
TCAGCAGTGGATTTTGACTTATTAGATCCCATACAGGGAATTTCTTACGT11.9230769230769231No Hit
CCTGTGCTTACGTCCCATCAGTATCTGTGACTAATTCTTAATCATCATCA11.9230769230769231No Hit
GAAGGAGATGATGACGTGGCCGGCTAGATCATCGCCGCAAAACACGAGGA11.9230769230769231No Hit
TGATCTTCAAACCCATCTCGAATCCTTACAAATTTTCTCAAGATTTAAAA11.9230769230769231No Hit
GTTCAACAACTAATTTGCGTCACTTTTCTTAAAGGATACAAACGTCAGTT11.9230769230769231No Hit
GCGAAGGTAAACCGACGATGATGATGACGCGTAGCCGTGGAGGTCAACAA11.9230769230769231No Hit
GCATCCTCATCCACGTCATTGTCATTGTCCTGGTCATCGTCATTCTCGTT11.9230769230769231No Hit
TGCCATGAAATATTTGCTGCAAAAGAAGGCTGCTAAGCCAAGATTTTTGA11.9230769230769231No Hit
TATTTTGTAGATATTTTGTCTCCAATGCTTACGTAATATTCTTGCACTGT11.9230769230769231No Hit
TTTGGCTTTGTATCTTCTAACAAGGAAATACTACTTAGGCTTATAAGATC11.9230769230769231No Hit
AATGTTATCATGTCTTACATGTTAGTGAAATAAAAAAATTGAAGTGATAA11.9230769230769231No Hit
GTTCATGGGAATGTCACTTCTGTAATGTGTATCAGTGTATGACGGATTTG11.9230769230769231No Hit
TACTTACGTGTTTTGAAGGTTGGGCCGTTAGCTGCTTACTACCAAATACC11.9230769230769231No Hit
AAAAGCCTTAGCGTGTATGATGACATTACAAAAGAAAAGGTATCACGAAG11.9230769230769231No Hit
TGTATATTTACGTTTTGATTTTATAATAAGAATGCAACTGTAATGTTAGG11.9230769230769231No Hit
TCGCCAGATAATGTCATGATTTTCTATATGCCATAATGATTATGTCAGCC11.9230769230769231No Hit
GCGTCCCTGAATACCCGCATGGATACAAAGGTAGCACTAGAATTGAGTAG11.9230769230769231No Hit
GCCGGGTCCCATGTAATCCATTTCCAACAAATGAGCCAGCGAACACGTCC11.9230769230769231No Hit
GGTGCAGTCGAGTATAGCTGGTTCATAGCCTGTCTGGTACTAGCCCTGTT11.9230769230769231No Hit
AAACAACACATGGTCTCTCCAAATTTTACGACATAGCATCATCTTCTGTA11.9230769230769231No Hit
CATACACATGACATAAGGTCATATTCGCCTCCAAAACACTAACCAAGCCT11.9230769230769231No Hit
TTTAAGTACCCATCCTTGTTATAATTTTTTTGTAAAAATTATGTGAGAAC11.9230769230769231No Hit
GCACCTCCCAGTATATCTCTGACAATCCCCGAGTCGTGCATTGACCCGTC11.9230769230769231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGC50.070.04
ATCCCCG50.070.013
TAGAAAC50.070.060
ACTGCGA50.070.032
ACAAAAG50.070.051
AAACTCA50.070.044
AAACTAC50.070.063
AACAAAA50.070.050
CCGGAAA50.070.017
GAAACTA50.070.062
AGCCAAA50.070.038
GAAATAA50.070.020
AATTTAT50.070.01
AACTGCG50.070.031
CCAAAAA50.070.040
CCCGGAA50.070.016
CAACTGC50.070.030
GGCAACT50.070.028
TAACGGC50.070.024
CATCCCC50.070.012
Produced by FastQC (version 0.11.5)