FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_coldna_daplib_itru-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_coldna_daplib_itru-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295527
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC2650.021.13203470
GAGCACC12800.019.14058970
TGGGGGG2803.914465E-916.2503241
TATGCCG4100.016.21948248
GCCGTCT2755.516813E-815.27269851
TGCCGTC3957.2759576E-1215.06326350
CGTATGC4900.014.99997246
TTCACGC1600.009099813.12497546
GTATGCC5103.6379788E-1213.03919147
ATGCCGT4952.5465852E-1112.72724949
AAGAGCC17050.012.72724870
CTGAACG3003.4368048E-511.66664570
TCCATCG6251.0913936E-1111.19997970
CACGAGC5005.140464E-911.19997870
TCGCGTA2200.00567202211.13634340
TCGGAAC6151.0004442E-1010.81298870
CGGAAGC19200.010.20831570
CTCGTAT5855.4496923E-910.17092144
GATTCCC8950.010.16757970
CGTCTGC8657.2759576E-129.30634170
Produced by FastQC (version 0.11.5)