FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_coldna_daplib_dapv2-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_coldna_daplib_dapv2-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4174332
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGCT4050.027.65367114
CAGCCCG4350.026.551424
GCGCTTC4350.026.55110216
CCTGCGC4300.026.045913
CCAGTCC16350.023.11927870
CCCGGAC5100.022.64687
GACCTGC5000.021.69949211
TGCGCTT5600.020.62451615
CCGGGGT3500.020.0002552
AGCCCGG5900.019.5760485
CGCTTCA5950.019.4113117
CCGGACC6500.018.8459368
CTGAACG8050.018.69566270
ATCGGGG11750.017.8723542
GCGGCGT3153.6379788E-1217.77852
CGGGGTG5800.017.50021753
ACCTGCG7550.017.15191712
TGCCGGG3551.8189894E-1216.76057250
GCCCGGA7350.016.6664776
GTATCGG23900.016.54812640
Produced by FastQC (version 0.11.5)