FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_coldna_daplib_dapv1-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_coldna_daplib_dapv1-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566190
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5000.022.39991446
CCAGTCC10500.022.33368170
GTATGCC5050.022.17813145
CCGGGTG1859.982614E-920.81072851
CGTATGC5250.019.99992244
GCCGTCT2453.092282E-1119.99992249
TGCCGTC3500.019.99992248
CTGAACG4800.019.68780770
ATGCCGT4800.019.68742447
CGGGGTC1150.001008505618.26079851
TGCCGGC1602.6095318E-517.49993148
ATGCCGG2301.2093915E-716.73906547
AGTCACC6250.016.24025370
CTCGTAT7200.016.04160542
CACGTCG6450.015.7366870
AAGAGCC25350.015.46375370
GCCGGGT2503.1210584E-715.399940550
AACTCCC7950.014.96878770
TCACAGG7550.014.37108570
TGCCGGG2303.7338275E-513.69559945
Produced by FastQC (version 0.11.5)