FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_coldna_daplib_dapv1-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_coldna_daplib_dapv1-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566190
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTCC10500.022.33368170
TATGCCG4950.021.91910646
GTATGCC5000.021.69991745
CCGGGTG1859.982614E-920.81072851
TGCCGTC3400.019.55874848
CGTATGC5200.019.51915444
CTGAACG4850.019.48483870
ATGCCGT4700.019.36162847
GCCGTCT2405.0386006E-1018.9582649
CGGGGTC1150.001008505618.26079851
TGCCGGC1602.6095318E-517.49993148
ATGCCGG2301.2093915E-716.73906547
AGTCACC6200.016.37122370
CTCGTAT7000.015.99993742
GGCGCCG1350.00294762315.55579965
AACTCCC7950.015.40904670
GCCGGGT2503.1210584E-715.399940550
AAGAGCC25700.015.38934470
CACGTCG6600.015.37902870
TGCCGGG2302.2186232E-615.21733245
Produced by FastQC (version 0.11.5)