FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_coldna_daplib_dapv1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_coldna_daplib_dapv1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAAGAAGAAGGTTGGTTAGTGTTTTGGAGTCGAATATGACTTGATCTC13.8461538461538463No Hit
CATACCTTGTATCCATGCGCTTCATATTCGCATGGAGTTCGCTCCCAGAA13.8461538461538463No Hit
ACAAACCAGAAACCACTATATGTGCTCCGGCTCCCCCTTCGTAACTCCAA13.8461538461538463No Hit
CCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGAGATCGGAAG13.8461538461538463No Hit
TTATGTCATCGCCACTCGCTTTGCCATCATCAACTAAGTCGGAACATTAT13.8461538461538463No Hit
CTTTTATGTCAAAACCAATTAACATAAAAGAGATCGGAAGAGCACACGTC13.8461538461538463No Hit
TTAGGCTTACAAGATCGGGTTGCGGTTTAAGTTGTTATAGATCGGAAGAG13.8461538461538463No Hit
CTTGGTAGAAGACACAAAGCCAAAGCCTCATATGGACCTTAACTACACCA13.8461538461538463No Hit
AGTGTTTGTGAGGTCTTATTCCGATTAAGCTTGGTAGAAGATCGGAAGAG13.8461538461538463No Hit
CTAACTCAGTTAAGTGAGAATAGGCAGACTTTGCTATTTAGTGCCACTTT13.8461538461538463No Hit
TTTATGTGACACAAAGGCTACTAGCCAGGCGACTTTGCTTGCCGATTCGG13.8461538461538463No Hit
AATCTATCGGGTCCGAGGAATCGTCGACCAGGACGAGGAATCGTCGACCG13.8461538461538463No Hit
CTTACGCAATGCACATCTTGTGTGAACAGCGTAGTCCTTACACACATTGC13.8461538461538463No Hit
ACTCCAAAACACTAACCAACCTTCTTCTTGCTTCTCAAGGCTTTCATGGT13.8461538461538463No Hit
GTCATCAGCTCGAGTTGCCTACGTGCCTGCCCTTTGTACACCCCGCCCGT13.8461538461538463No Hit
AAAGTGTTACTCGTAAAAAAATCTAGCTAGTAGATATTTCATCGCCATAT13.8461538461538463No Hit
AGTCGAATATGACTTGATGTCATGTGTATGATTGAGTATAACAACTTAAA13.8461538461538463No Hit
CCATTCTTCTTCCTAGTGACCTAGGAGTTGGGTTCGTGACATTTTTTCTT13.8461538461538463No Hit
AGTATTTATCAAATCTGTTCATATCTAACGGCACGCTATTGGAGCGAATG13.8461538461538463No Hit
TTGGCTACACCATGAAAGCTTTGAGAAGCTAGAAGAAGGTAGGTTAGTGA13.8461538461538463No Hit
TTATACTCAATGATACACATGACGTCAAGTCATATTCGACTCCAAAACAC13.8461538461538463No Hit
CGTTGAGAAGCAAGAAGAAGGTTGTTTAGTGTTTTAAAGTCGAATATGAC13.8461538461538463No Hit
CGACGGATCGCATGGCCGCTGTGCTGGCGAAGCATCATTCAAATTTCTGC13.8461538461538463No Hit
TAGCAGAAGGTTGGTTAGTGTTTTGGAGTCGAATTAGCCGTGATGTCATG13.8461538461538463No Hit
GGGTTGCGGTTTAAGTTCTTATACTCAATAATACACATGACATCAAGTCA13.8461538461538463No Hit
ATACTCAATCATACACATGACATCAAGTCATATTCGACTCCAAAACACTA13.8461538461538463No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)