FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_c24dna_daplib_itru-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_c24dna_daplib_itru-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772154
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG95640.5396822172339424TruSeq Adapter, Index 6 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG21250.046.9409342
TCGGGGG21900.046.3467943
AATCGGG22550.044.07959441
GAATCGG23350.043.46872340
AGAATCG25900.040.6754639
CGGGGGG24750.039.31292744
CGGAGAA40200.037.5246836
GGAGAAT37900.036.4773837
CCGGAGA45150.036.0463235
TCCGGAG47300.035.81376634
CTCCGGA48750.034.67674333
GAGAATC31650.033.9492738
ACTCCGG49500.033.6563932
CACTCCG52600.031.53975931
TAGGGGG3000.030.33317643
TCACTCC58150.028.46932830
ATGGGGG6250.027.43985642
GTCACTC60600.027.26058629
AGTCACT64500.025.88358328
ATAGGGG4950.025.45441242
Produced by FastQC (version 0.11.5)