FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_c24dna_daplib_dapv2-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_c24dna_daplib_dapv2-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435629
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATGGGGGTT26910.11048480700467929TruSeq Adapter, Index 13 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG12800.048.94541
ATCGCGG7450.046.5097742
CCGGGTG5650.045.222853
TGCGGGG7400.044.93306751
ATCGGGG8700.044.25258642
CAGAAAT116050.042.5848237
AATCGGG13850.042.20189341
AATGGGG17750.041.80254441
GCGGGGT10600.041.60521352
TCAGAAA130850.040.01502636
AGAAATC64200.039.633738
TTCAGAA143600.039.53317335
TATGGCG5200.039.03901348
CGGGGTG11650.038.75670653
CGCGTAT4000.038.4997544
AATGGCG12850.038.40442341
CCGGCGT2850.038.07149552
CCGGGGT4600.038.04479652
AAATCGG19150.037.83265340
ATGGGGG13800.037.7896142
Produced by FastQC (version 0.11.5)