FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_c24dna_daplib_dapv1-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_c24dna_daplib_dapv1-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930405
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG6900.042.60875751
TGCGGGG3750.040.13443449
TCCATGG26450.037.05205537
CATGGGG12000.037.04268339
GCGGGGT7700.036.3646350
GTTCCAT46500.036.35583535
CCATGGC11900.035.29508638
TTCCATG38250.035.0467136
AGTTCCA57850.034.30517634
CCGGGGT2650.033.0197750
ATGGGGG9250.032.9198240
CATGGCG11750.031.87321339
CAGTTCC70750.031.51241333
TCCAGGG6000.031.50086237
ACAGTTC74150.030.35068732
TGCCGGG3050.029.83688448
GGCGGGG9050.029.7798249
CCATGGG17500.029.40080538
CATCGCG3300.028.63714839
CACAGTT80850.028.2684431