FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_c24dna_daplib_dapv1-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_c24dna_daplib_dapv1-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930405
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG6800.042.20594451
TGCGGGG3700.039.7308249
CATGGGG11900.037.35396639
TCCATGG26600.036.97469737
GTTCCAT46650.036.4640235
CCATGGC12000.035.29263338
GCGGGGT7750.035.22677250
TTCCATG38300.035.00095736
AGTTCCA58050.034.4281634
CCGGGGT2650.033.0197750
ATGGGGG9200.032.71828540
CATGGCG11850.031.89960939
CAGTTCC70950.031.67023733
TCCAGGG6000.031.50086237
ACAGTTC74450.030.46344432
TGCCGGG3050.029.83688448
CCATGGG17350.029.6549938
GGCGGGG9100.029.2315749
CATCGCG3400.028.82431839
CACAGTT81150.028.37958531
Produced by FastQC (version 0.11.5)