FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_abdna_ablib_itru-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_abdna_ablib_itru-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2771713
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT43440.15672618341076439TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8650.030.75120750
ATGCCGT11550.029.39371149
TATGCCG14900.027.71790948
GCCGTCT6550.026.7173551
CACCGCG6000.026.2502770
GTATGCC18100.023.97771547
TGCCGTG5050.021.48498350
GCCGTCG4100.021.341351
TGCCGGG2001.0713848E-920.99983850
CCGTCTT10100.020.44575552
CGTATGC21000.020.33317646
CCGGGGT1408.257419E-620.00020652
TGGCGTC3500.019.99984650
ATGCCGG4150.019.39743849
TGCCGGC2659.458745E-1118.49042350
GTATGGC6650.017.894647
CCGTCGT5100.017.8433252
TATGGCG6250.017.35986548
CCGGCGT1653.3981847E-516.96987252
TCGTATG28350.016.91344845
Produced by FastQC (version 0.11.5)