FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_abdna_ablib_dapv1-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_abdna_ablib_dapv1-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522322
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT3400.022.64680949
TGCCGTC5150.021.74733248
TGCCGGC2002.445995E-819.24978848
GCGTCGT1651.5948626E-619.09069850
GGCTGGG950.007213392318.4208498
ATGCCGT7700.017.72707647
CGCGGAA1755.622799E-516.0007761
TATGCCG9600.015.67690946
TATGGCG5050.014.55429546
ATGCCGG4151.8189894E-1114.33747570
CCGTCTT6450.014.10837150
CCGGCGT1750.001018622913.99984651
GGGCGAG1800.00126010713.61096115
GAGCACC16350.013.48635770
GCCGGCT2102.654161E-413.33318649
GTCAATG25100.013.247128570
CGGGGTG2153.2385372E-413.02311251
TGCCGGG1900.001893347712.8948570
CCAGTCC9100.012.69241970
CACGTCG4255.5806595E-912.35304970
Produced by FastQC (version 0.11.5)