FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_abdna_ablib_dapv1-2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_abdna_ablib_dapv1-2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522322
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT3550.022.67580649
TGCCGTC5250.022.66641648
TGCCGGC2101.9681465E-919.99977948
GCGTCGT1702.1220458E-618.52920750
ATGCCGT7750.018.51592447
GGCTGGG1000.00969410117.4998078
TATGCCG9600.016.40606946
CGCGGAA1755.622799E-516.0007761
CGGGGTG2151.9775003E-514.65100151
TATGGCG5050.014.55429546
CCGTCTT6500.014.538300550
ATGCCGG4202.1827873E-1114.16679170
CCGGCGT1800.00126010713.61096150
GGGCGAG1800.00126010713.61096115
GCCGTCG3102.387951E-713.54823949
GTCAATG24950.013.467051570
GAGCACC16400.013.4452470
GCCGGCT2102.654161E-413.33318649
CACACGG5850.013.16250970
TGCCGGG1900.001893347712.8948570
Produced by FastQC (version 0.11.5)