FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_abdna_ablib_dapv1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_abdna_ablib_dapv1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACATCAATTCATATTCGACTCCAACACCCTATCCAACCTTCTTCTTGCT17.142857142857142No Hit
ATGGTTCTAGTTGTAATACTCTCTAATACAAATGACAGCTAGTAATAATT17.142857142857142No Hit
CTGACATCGATCAAGGAATCTCTGTCTTAAAGATAAGAGGACATTTAGTA17.142857142857142No Hit
CCCATGATCTGTCTGAAAATGCGGTGATATTATTGTGTTCAGAACGGTAG17.142857142857142No Hit
CGTCGTCCTAATCCCACAGGATTCACATCGTCCCACTTCAGTGTAGGCGG17.142857142857142No Hit
ATGTATAGCTGAATCTCTGATTGTTTGATGGAAATCCAAGATGCATATGA17.142857142857142No Hit
ATATCTATGTACCTAATATAGATATAGATGGTAAGAGCACACGTCTGACC17.142857142857142No Hit
TAGGGCTATCGAGTATACAACTTCCTGGATTAGGCATCATTGCATTGATT17.142857142857142No Hit
CAATATCACTTGACTTCTCAATTAGTGAGATCGGAAGAGCACACGTCTGA17.142857142857142Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGTAATCTTTAAGCTCAAAGGTGAAGCTAGAGCGGAAGAGCACACGTC17.142857142857142No Hit
CACCATTGCAACAAGTTGCTCAAGTCCTTAAGCAATCAATTTCTCCTGTT17.142857142857142No Hit
CTCCTTTTGTCCTCGCATGAGCTAGTAGAGGGTGCAGGTTATACCAATCG17.142857142857142No Hit
CGTCAGTATCGCTGCGGGCTTCCACCAGAGTTTCCTCTGGCTTTACCCCG17.142857142857142No Hit
ATCCAGTTTTCGCCGGACATAGTCGCAACTCAAAGAGAGAGAGGAGAGAT17.142857142857142No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)