FastQCFastQC Report
Thu 6 Dec 2018
HG3LMAFXY_n01_WRKY40_col_b-2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHG3LMAFXY_n01_WRKY40_col_b-2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3216646
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC9000.015.55563370
GCGTTGC2600.001596759810.76928370
CCCCGGG2650.00187132610.56608970
CGGAAGC12850.09.53312170
GGCATCG4056.792002E-59.5062270
CACTTAG27500.09.41822870
CGTCTGC5805.3389704E-79.05176870
GCCCAGG15800.08.6392821
AGGCATG9353.6379788E-118.60966870
CCGTCTG4502.1375927E-48.55559768
AAGAGCC15300.08.46409470
CAGTCAC45800.08.02405669
GTCACTT38950.07.907613370
CACACGG4000.00609755337.87503870
TAGGCAT13350.07.86520768
TCACTTA37400.07.86100170
GCGGAAC4905.3500466E-47.857059532
TTAGGCC5451.9700971E-47.7064670
GGCATGG7755.1671486E-77.67745770
GATAGGG4150.0084789047.5902832